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AB227427

Anti-ARPC1B antibody

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(1 Publication)

Rabbit Polyclonal ARPC1B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARPC1B.

View Alternative Names

ARC41, ARPC1B, Actin-related protein 2/3 complex subunit 1B, Arp2/3 complex 41 kDa subunit, p41-ARC

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARPC1B antibody (AB227427)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARPC1B antibody (AB227427)

Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for ARPC1B using ab227427 (green) at 1/500 dilution in ICC/IF. Counterstained with Hoechst 33342 (blue).

Western blot - Anti-ARPC1B antibody (AB227427)
  • WB

Supplier Data

Western blot - Anti-ARPC1B antibody (AB227427)

10% SDS PAGE

All lanes:

Western blot - Anti-ARPC1B antibody (ab227427) at 1/500 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ARPC1B. The exact immunogen used to generate this antibody is proprietary information.

O15143

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARPC1B also known as Actin-related protein 2/3 complex subunit 1B weighs around 41 kDa. This protein forms a part of the Arp2/3 complex which is instrumental in actin filament branching and growth. Researchers have detected ARPC1B expression in various tissues including blood cells and the skin highlighting its diverse functional roles in different cell types.
Biological function summary

ARPC1B contributes significantly to cell movement and shape maintenance. The protein acts as a component of the Arp2/3 complex which always requires additional nucleation factors to perform its function. This complex drives the reorganization of the actin cytoskeleton influencing cellular processes like migration and phagocytosis. It modulates the interaction of actin with branching networks thereby ensuring dynamic cellular adaptability to different environments.

Pathways

ARPC1B plays a critical role in the regulation of actin cytoskeleton and focal adhesion pathways. Scientists have associated ARPC1B with the WASP and WAVE proteins which stimulate actin assembly. These interactions enable ARPC1B to facilitate precise signaling pathways important for cell migration and immune responses. The protein’s participation in these pathways illustrates its key function in maintaining cytoskeletal integrity and cellular communication.

ARPC1B has connections to severe combined immunodeficiency and Wiskott-Aldrich syndrome. These conditions often manifest through mutations affecting the actin cytoskeleton. ARPC1B’s relationship with the WASP protein highlights its involvement in regulating immune cell function and signaling. For individuals with deficiencies in ARPC1B these immune complications underline the importance of understanding its function in therapeutic contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 11741539, PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 11741539, PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC1B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34396443

2021

miR‑125a‑5p and miR‑7 inhibits the proliferation, migration and invasion of vascular smooth muscle cell by targeting EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Hualan Zhou,Sen Lin,Youdong Hu,Dianxuan Guo,Yun Wang,Xia Li
View all publications
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Product promise

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