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AB199114

Anti-ARPC4 antibody

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(1 Publication)

Rabbit Polyclonal ARPC4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARPC4.

View Alternative Names

ARC20, ARPC4, Actin-related protein 2/3 complex subunit 4, Arp2/3 complex 20 kDa subunit, p20-ARC

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC4 antibody (AB199114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC4 antibody (AB199114)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling ARPC4 using ab199114 at a 1/15 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC4 antibody (AB199114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC4 antibody (AB199114)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ARPC4 using ab199114 at a 1/15 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ARPC4. The exact immunogen used to generate this antibody is proprietary information.

P59998

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARPC4 protein also known as Actin-Related Protein 2/3 Complex Subunit 4 plays a significant role in the actin nucleation process. It is a component of the ARP2/3 complex which is essential for the formation of branched actin filament networks. ARPC4 has a molecular weight of approximately 20 kDa. It is ubiquitously expressed across various tissues indicating its fundamental role in cellular mechanisms.
Biological function summary

ARPC4 contributes to the dynamic remodeling of the actin cytoskeleton. It is an integral part of the ARP2/3 complex a multi-protein structure that facilitates actin filament branching promoting cell movement and shape changes. This complex is critical for processes like cell migration phagocytosis and intracellular trafficking. By aiding in these activities ARPC4 influences cellular responses to environmental signals and stress.

Pathways

Proteins related to cellular movement and structure rely significantly on actin network regulation. ARPC4 through the ARP2/3 complex participates in the regulation of the WAVE regulatory complex (WRC) and is involved in the actin-related pathways that include Rac1 and N-WASP. These pathways are key to transmitting signals that control cytoskeletal reorganization and influence cell polarity movement and division.

Aberrations in the actin filament network including those involving ARPC4 can contribute to pathological conditions. Research suggests that disruptions in ARPC4 function are linked to cancer metastasis due to its role in cell motility. Additionally ARPC4 has connections with neurological disorders through its interaction with proteins like N-WASP and Rac1 which are known to influence neural cell signaling and structure. Understanding the role of ARPC4 in these diseases may offer potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 70:123-135 PubMed36916928

2023

Cathepsin C promotes colorectal cancer metastasis by regulating immune escape through upregulating CSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Zhi Dang,Xiao-Jiao Chen,Jiao Yu,Shu-Hong Zhao,Xi-Ming Cao,Qing Wang
View all publications
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Product promise

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