Rabbit Polyclonal ARPC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARPC4 aa 1-50.
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Publications
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- Nature 611:365-3732022Extracellular fluid viscosity enhances cell migration and cancer dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaustav Bera,Alexander Kiepas,Inês Godet,Yizeng Li,Pranav Mehta,Brent Ifemembi,Colin D Paul,Anindya Sen,Selma A Serra,Konstantin Stoletov,Jiaxiang Tao,Gabriel Shatkin,Se Jong Lee,Yuqi Zhang,Adrianna Boen,Panagiotis Mistriotis,Daniele M Gilkes,John D Lewis,Chen-Ming Fan,Andrew P Feinberg,Miguel A Valverde,Sean X Sun,Konstantinos KonstantopoulosPubMed 36323783
- Oncology letters 15:2847-28542018Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Su,Siyang Wang,Yongxu Huo,Chunlei YangPubMed 29435011
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ARPC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P59998
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
ARC20, ARPC4, Actin-related protein 2/3 complex subunit 4, Arp2/3 complex 20 kDa subunit, p20-ARC
Recommended products
Rabbit Polyclonal ARPC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARPC4 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ARPC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P59998
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab217065 was affinity purified using an epitope specific to ARPC4 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ARPC4 protein also known as Actin-Related Protein 2/3 Complex Subunit 4 plays a significant role in the actin nucleation process. It is a component of the ARP2/3 complex which is essential for the formation of branched actin filament networks. ARPC4 has a molecular weight of approximately 20 kDa. It is ubiquitously expressed across various tissues indicating its fundamental role in cellular mechanisms.
- Biological function summary
ARPC4 contributes to the dynamic remodeling of the actin cytoskeleton. It is an integral part of the ARP2/3 complex a multi-protein structure that facilitates actin filament branching promoting cell movement and shape changes. This complex is critical for processes like cell migration phagocytosis and intracellular trafficking. By aiding in these activities ARPC4 influences cellular responses to environmental signals and stress.
- Pathways
Proteins related to cellular movement and structure rely significantly on actin network regulation. ARPC4 through the ARP2/3 complex participates in the regulation of the WAVE regulatory complex (WRC) and is involved in the actin-related pathways that include Rac1 and N-WASP. These pathways are key to transmitting signals that control cytoskeletal reorganization and influence cell polarity movement and division.
- Associated diseases and disorders
Aberrations in the actin filament network including those involving ARPC4 can contribute to pathological conditions. Research suggests that disruptions in ARPC4 function are linked to cancer metastasis due to its role in cell motility. Additionally ARPC4 has connections with neurological disorders through its interaction with proteins like N-WASP and Rac1 which are known to influence neural cell signaling and structure. Understanding the role of ARPC4 in these diseases may offer potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ARPC4 antibody (ab217065)
Lysates were prepared using NETN lysis buffer.
All lanes: Western blot - Anti-ARPC4 antibody (ab217065) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 15 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: Jurkat whole cell lysate at 15 µg
Lane 4: TCMK-1 whole cell lysate at 15 µg
Lane 5: NIH3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 30s
Immunoprecipitation - Anti-ARPC4 antibody (ab217065)
Detection of ARPC4 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab217065 at 6 μg/mg lysate for IP (Lane 1), and at 0.4 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 75 seconds.
All lanes: Immunoprecipitation - Anti-ARPC4 antibody (ab217065)
Predicted band size: 20 kDa
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