AB217065

Anti-ARPC4 antibody

Name: Anti-ARPC4 antibody (ab217065) | Abcam
Brand: Abcam Limited
SKU: AB217065

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(3 Publications)

Rabbit Polyclonal ARPC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ARPC4 aa 1-50.

View Alternative Names

ARC20, ARPC4, Actin-related protein 2/3 complex subunit 4, Arp2/3 complex 20 kDa subunit, p20-ARC

2 Images

Supplier Data

Immunoprecipitation - Anti-ARPC4 antibody (AB217065)

Detection of ARPC4 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab217065 at 6 μg/mg lysate for IP (Lane 1), and at 0.4 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Immunoprecipitation - Anti-ARPC4 antibody (ab217065)

Predicted band size: 20 kDa

false

Supplier Data

Western blot - Anti-ARPC4 antibody (AB217065)

Lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ARPC4 antibody (ab217065) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 15 µg

Lane 4:

TCMK-1 whole cell lysate at 15 µg

Lane 5:

NIH3T3 whole cell lysate at 15 µg

Predicted band size: 20 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ARPC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P59998

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab217065 was affinity purified using an epitope specific to ARPC4 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARPC4 protein also known as Actin-Related Protein 2/3 Complex Subunit 4 plays a significant role in the actin nucleation process. It is a component of the ARP2/3 complex which is essential for the formation of branched actin filament networks. ARPC4 has a molecular weight of approximately 20 kDa. It is ubiquitously expressed across various tissues indicating its fundamental role in cellular mechanisms.

Biological function summary

ARPC4 contributes to the dynamic remodeling of the actin cytoskeleton. It is an integral part of the ARP2/3 complex a multi-protein structure that facilitates actin filament branching promoting cell movement and shape changes. This complex is critical for processes like cell migration phagocytosis and intracellular trafficking. By aiding in these activities ARPC4 influences cellular responses to environmental signals and stress.

Pathways

Proteins related to cellular movement and structure rely significantly on actin network regulation. ARPC4 through the ARP2/3 complex participates in the regulation of the WAVE regulatory complex (WRC) and is involved in the actin-related pathways that include Rac1 and N-WASP. These pathways are key to transmitting signals that control cytoskeletal reorganization and influence cell polarity movement and division.

Aberrations in the actin filament network including those involving ARPC4 can contribute to pathological conditions. Research suggests that disruptions in ARPC4 function are linked to cancer metastasis due to its role in cell motility. Additionally ARPC4 has connections with neurological disorders through its interaction with proteins like N-WASP and Rac1 which are known to influence neural cell signaling and structure. Understanding the role of ARPC4 in these diseases may offer potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ARPC4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4491 PubMed40368919

2025

Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.

Applications

Unspecified application

Species

Unspecified reactive species

Beth L Woodward,Sudipta Lahiri,Anoop S Chauhan,Marcos Rios Garcia,Lucy E Goodley,Thomas L Clarke,Mohinder Pal,Angelo Agathanggelou,Satpal S Jhujh,Anil N Ganesh,Fay M Hollins,Valentina Galassi Deforie,Reza Maroofian,Stephanie Efthymiou,Andrea Meinhardt,Christopher G Mathew,Michael A Simpson,Heather C Mefford,Eissa A Faqeih,Sergio D Rosenzweig,Stefano Volpi,Gigliola Di Matteo,Caterina Cancrini,Annarita Scardamaglia,Fiona Shackley,E Graham Davies,Shahnaz Ibrahim,Peter D Arkwright,Maha S Zaki,Tatjana Stankovic,A Malcolm R Taylor,Antonina J Mazur,Nataliya Di Donato,Henry Houlden,Eli Rothenberg,Grant S Stewart

Nature 611:365-373 PubMed36323783

2022

Extracellular fluid viscosity enhances cell migration and cancer dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Kaustav Bera,Alexander Kiepas,Inês Godet,Yizeng Li,Pranav Mehta,Brent Ifemembi,Colin D Paul,Anindya Sen,Selma A Serra,Konstantin Stoletov,Jiaxiang Tao,Gabriel Shatkin,Se Jong Lee,Yuqi Zhang,Adrianna Boen,Panagiotis Mistriotis,Daniele M Gilkes,John D Lewis,Chen-Ming Fan,Andrew P Feinberg,Miguel A Valverde,Sean X Sun,Konstantinos Konstantopoulos

Oncology letters 15:2847-2854 PubMed29435011

2018

Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Su,Siyang Wang,Yongxu Huo,Chunlei Yang

View all publications

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Product promise

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Anti-ARPC4 antibody

Immunoprecipitation - Anti-ARPC4 antibody (AB217065)

Western blot - Anti-ARPC4 antibody (AB217065)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise