Anti-ARPC5/p16 ARC antibody [EP1551Y] is a rabbit recombinant monoclonal antibody that is used to detect ARPC5/p16 ARC in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, Antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1551Y is the most widely used clone for ARPC5/P16 ARC on the market
- Specificity confirmed with ARPC5 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunocytochemistry-immunofluorescence-img24688.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)")
![Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunoprecipitation-img16023.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)")
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img10379.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)")
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img101095.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77353.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)")
Publications
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- Cell death & disease 14:3212023Clinical-grade human umbilical cord-derived mesenchymal stem cells improved skeletal muscle dysfunction in age-associated sarcopenia mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Wang,Bichun Zhao,Jinglei Zhai,Ailin Wang,Ning Cao,Tuling Liao,Ruyu Su,Lijuan He,Yanhua Li,Xuetao Pei,Yali Jia,Wen YuePubMed 37173309
- Frontiers in immunology 14:11859212023Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Li,Haifeng Ye,Tie Su,Chuan Hu,Yaoqi Huang,Xinxin Fu,Zhisheng Zhong,Xuelian Du,Yuehui ZhengPubMed 37228612
- Stem cell research & therapy 14:1212023Comparison of therapeutic effects of mesenchymal stem cells derived from superficial and deep subcutaneous adipose tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoki Ishiuchi,Ayumu Nakashima,Satoshi Maeda,Yoshie Miura,Kisho Miyasako,Kensuke Sasaki,Toshio Uchiki,Ayano Sasaki,Shogo Nagamatsu,Naoki Nakao,Masataka Nagao,Takao MasakiPubMed 37143086
- Drug design, development and therapy 17:1289-13022023Integrating Network Pharmacology and Experimental Validation to Explore the Pharmacological Mechanism of Astragaloside IV in Treating Bleomycin-Induced Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu Yuan,Biao Zuo,Si-Cong Zhou,Meng Wang,Kai-Yue Tan,Zhi-Wei Chen,Wen-Fu CaoPubMed 37138582
- Journal of nanobiotechnology 21:1352023MicroRNA-19b-3p dysfunction of mesenchymal stem cell-derived exosomes from patients with abdominal aortic aneurysm impairs therapeutic efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxiao Zhang,Xiaoran Huang,Tucheng Sun,Linli Shi,Baojuan Liu,Yimei Hong,Qing-Ling Fu,Yuelin Zhang,Xin LiPubMed 37101174
- International journal of molecular sciences 24:2023Effect of Lacking ZKSCAN3 on Autophagy, Lysosomal Biogenesis and Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Min Li,Jun-Hao Wen,Ze-Sen Feng,Yun-Shan Wu,Dong-Yi Li,Shan Liang,Dan Wu,Hong-Luan Wu,Shang-Mei Li,Zhen-Nan Ye,Chen Yang,Lin Sun,Ji-Xin Tang,Hua-Feng LiuPubMed 37175493
- Nutrients 15:2023Sesamin Metabolites Suppress the Induction of Cellular Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChie Araki,Daisuke Takemoto,Yoshinori Kitagawa,Norifumi Tateishi,Tomohiro Rogi,Takayuki Izumo,Shimpei Kawamoto,Hiroshi Shibata,Eiji Hara,Masaaki NakaiPubMed 37049468
- Alzheimer's research & therapy 15:532023Attenuation of Alzheimer's brain pathology in 5XFAD mice by PTH, a peptide of parathyroid hormone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Lei Xiong,Lingling Yao,Jinxiu Pan,Emily Arzola,Xiaojuan Zhu,Lin Mei,Wen-Cheng XiongPubMed 36918976
- Journal of orthopaedic surgery and research 18:1982023Metformin regulates chondrocyte senescence and proliferation through microRNA-34a/SIRT1 pathway in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiju Yan,Wenjing Dong,Zhirui Li,Junqiang Wei,Tao Han,Junliang Wang,Feng LinPubMed 36915137
- Molecular therapy. Nucleic acids 32:94-1102023Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang Wu,Wei Zhou,Jingwei Jiang,Mengyue Wang,Jinwei Zhang,Jing Yang,Qu Tang,Huawen Liu,Deming Liu,Wei Xu,Julia Li Zhong,Li Yang,Mingxing LeiPubMed 37020681
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Not recommended | Tested |
| Mouse | Expected | Tested | Expected | Not recommended | Tested |
| Rat | Expected | Expected | Expected | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes We have obtained results that indicate IHC-P is unsuitable for mouse and rat species. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/70 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
ARC16, ARPC5, Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC
Recommended products
Anti-ARPC5/p16 ARC antibody [EP1551Y] is a rabbit recombinant monoclonal antibody that is used to detect ARPC5/p16 ARC in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, Antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1551Y is the most widely used clone for ARPC5/P16 ARC on the market
- Specificity confirmed with ARPC5 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1551Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific to the p16 ARC protein, database link: O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been cited over 158 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been confirmed by testing in knockout samples.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has 5 independent reviews from customers.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) specifically detects ARPC5/p16 ARC (UniProt ID: O15511; Molecular weight: 16kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1551Y - Anti-ARPC5/p16 ARC antibody [EP1551Y] - BSA and Azide free ab220800.
Antibody clone EP1551Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 750 (Alexa Fluor® 488 Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320122, Alexa Fluor® 647 Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320123, Alexa Fluor® 555 Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320124, Alexa Fluor® 594 Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320125, PE Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320126, APC Anti-ARPC5/p16 ARC antibody [EP1551Y] ab320127, Alexa Fluor® 750 Anti-ARPC5/p16 ARC antibody [EP1551Y] ab321644).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARPC5 also known as p16 ARC is a protein with a molecular weight of roughly 16 kilodaltons. It localizes largely in the cytoplasm and expresses in many cell types across species. The ARPC5 protein is a subunit of the Arp2/3 complex a vital component responsible for actin nucleation and branching during cytoskeleton remodeling. Researchers often examine ARPC5 through techniques such as western blot and immunohistochemistry using specific antibodies like anti-p16 or ARPC5 anticuerpo to identify its presence and distribution.
- Biological function summary
The ARPC5 protein is essential for effective actin polymerization making it an important contributor to cell motility and structure. As part of the Arp2/3 complex it interacts with other proteins to stimulate the growth of new actin filaments. This activity is important in processes like endocytosis phagocytosis and cell migration. Furthermore ARPC5 plays a role in maintaining cellular homeostasis and is involved in cellular signaling pathways.
- Pathways
Arpc5 influences the regulation of the actin cytoskeleton which intersects with pathways associated with cell movement and morphogenesis. It prominently relates to the WAVE complex working synergistically to regulate actin dynamics. ARPC5's involvement in the Rac1-WAVE-Arp2/3 pathway highlights its relationship with Rac1 a small GTPase known for regulating the cytoskeleton and cellular functions like adhesion and migration.
- Associated diseases and disorders
Imbalances in ARPC5 function can link to cancer progression and neurodegenerative diseases. Aberrant activity of the Arp2/3 complex which involves ARPC5 associates with increased cell invasion and metastasis in cancer. Additionally disruptions in ARPC5 activity connect to neurodegenerative conditions where cytoskeletal arrangement alterations play a role. The protein's interaction with others in the complex such as Rac1 further deepens its involvement in these diseases signaling potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunocytochemistry-immunofluorescence-img24688.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
![Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunoprecipitation-img16023.jpg "Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.
Lane 1 (input): Human fetal brain tissue lysate (10μg)
Lane 2 (+): ab51243 + human fetal brain tissue lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab51243 in human fetal brain tissue lysate.
For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img10379.jpg "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: p16 ARC knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab51243 observed at 16 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab51243 was shown to specifically react with p16 ARC when p16 ARC knockout samples were used. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE. ab51243 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Predicted band size: 16 kDa
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img101095.jpg "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: SKBR-3 whole cell lysate at 20 µg
Lane 3: MCF-7 whole cell lysate at 20 µg
Lane 4: HepG2 whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77353.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img143084.jpg "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution
Lane 1: Human fetal brain tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img106787.jpg "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: Mouse brain tissue lysate at 10 µg
Lane 3: Rat brain tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57585.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img143084.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
![Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--western-blot-img106787.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243), expandable thumbnail](https://content.abcam.com/products/images/arpc5-p16-arc-antibody-ep1551y-ab51243--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57585.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)")
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