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AB249510

Anti-ARPC5L antibody [EPR10274] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ARPC5L antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Actin-related protein 2/3 complex subunit 5-like protein, Arp2/3 complex 16 kDa subunit 2, ARC16-2, ARPC5L

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)

This data was developed using ab169763, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ARPC5L with ab169763 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)

This data was developed using ab169763, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells labeling ARPC5 with ab169763 at 1/250 (red) with DAPI staining (blue).

Flow Cytometry (Intracellular) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)

This data was developed using ab169763, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized MOLT4 cells with ab169763 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)
  • WB

Supplier Data

Western blot - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (AB249510)

This data was developed using ab169763, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARPC5L antibody [EPR10274] (<a href='/en-us/products/primary-antibodies/arpc5l-antibody-epr10274-ab169763'>ab169763</a>) at 1/1000 dilution

Lane 1:

U87-MG lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Lane 3:

MOLT4 lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10274

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249510 is the carrier-free version of ab169763.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARPC5L also known as Actin-Related Protein 2/3 Complex Subunit 5-Like is a smaller subunit of the ARP2/3 complex with a molecular mass around 16 kDa. This protein acts mechanically as an important regulator in the nucleation of new actin filaments aiding in the branching of the actin cytoskeleton. Scientists detect ARPC5L expression across diverse human tissues pointing to its broad role in cellular dynamics.
Biological function summary

ARPC5L aids actin filament dynamics as part of the ARP2/3 complex. This complex essential for cellular shape and motility helps drive the formation of lamellipodia and filopodian important structures in cell migration. Its function in remodeling the cytoskeleton makes it important in processes such as endocytosis and intracellular trafficking contributing to normal cell function and response to external stimuli.

Pathways

ARPC5L works within the cytoskeletal remodeling and cell motility pathways. Within the Wnt signaling pathway it plays a role in the regulation of cytoskeletal organization letting cells respond to signaling cues. The protein associates with others like ARPC1 and ARPC2 to facilitate these structural changes highlighting its importance to cellular adaptability and movement.

ARPC5L has connections to cancers including breast and colorectal cancer. Abnormal regulation or mutation of the ARP2/3 complex can lead to altered cell migration and invasiveness contributing to cancer progression. The protein's interaction with elements of the Rho family such as RhoA and Rac1 integrates it into processes often hijacked in malignancies impacting how cells move invade and adhere.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
See full target information ARPC5L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com