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AB235421

Anti-Arpin antibody

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(2 Publications)

Rabbit Polyclonal ARPIN antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARPIN aa 1 to C-terminus.

View Alternative Names

C15orf38, ARPIN, Arpin, Arp2/3 inhibition protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arpin antibody (AB235421)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arpin antibody (AB235421)

Paraffin-embedded human kidney tissue stained for Arpin using ab235421 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arpin antibody (AB235421)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arpin antibody (AB235421)

Paraffin-embedded human colon cancer tissue stained for Arpin using ab235421 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Arpin antibody (AB235421)
  • WB

Supplier Data

Western blot - Anti-Arpin antibody (AB235421)

All lanes:

Western blot - Anti-Arpin antibody (ab235421) at 1/1000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARPIN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6K5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arpin also known as the Arp2/3 complex inhibitor plays an important role in actin dynamics. Mechanically Arpin acts by inhibiting the Arp2/3 complex which is essential for the nucleation of new actin filaments. This inhibition affects cell motility and migration. Arpin has a molecular mass of approximately 20 kDa and is ubiquitously expressed in various tissues including epithelial and nerve cells.
Biological function summary

Arpin regulates cell motility by controlling the formation of branched actin networks impacting processes like cell shape and movement. Arpin is not part of a larger protein complex but directly interacts with the Arp2/3 complex. Through this interaction it modulates the cytoskeleton's dynamic rearrangement affecting cellular responses to environmental signals.

Pathways

The inhibition of actin nucleation by Arpin influences the WAVE regulatory complex (WRC) and integrin signaling. These pathways are important for cell migration and adhesion. Arpin affects proteins such as Rac1 which is a part of the WRC pathway altering the actin network and impacting cell behavior.

Arpin's regulation of actin dynamics connects it to cancer metastasis and neurodegenerative conditions. Dysregulation of Arpin can lead to abnormal cell migration contributing to cancer spread. Additionally its role in actin polymerization links it to proteins like cofilin associated with neurodegenerative diseases due to cytoskeletal disruptions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors. Participates in an incoherent feedforward loop at the lamellipodium tip where it inhibits the ARP2/2 complex in response to Rac signaling and where Rac also stimulates actin polymerization through the WAVE complex. Involved in steering cell migration by controlling its directional persistence.
See full target information ARPIN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecules and cells 46:351-359 PubMed36921992

2023

RNA Editing Enzyme ADAR1 Suppresses the Mobility of Cancer Cells via ARPIN.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ji Park,Eunji Jeong,Eun Ji Lee,Hyeon Ji Choi,Bo Hyun Moon,Keunsoo Kang,Suhwan Chang

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology 45:769-775 PubMed30416079

2018

Arpin downregulation is associated with poor prognosis in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Rong Zhang,Hao Li,Wen-Quan Wang,Wei Jin,Jin-Zhi Xu,Hua-Xiang Xu,Chun-Tao Wu,He-Li Gao,Shuo Li,Tian-Jiao Li,Wu-Hu Zhang,Shuai-Shuai Xu,Quan-Xing Ni,Xian-Jun Yu,Liang Liu
View all publications
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