AB181758

Anti-ARRDC1 antibody

Name: Anti-ARRDC1 antibody (ab181758) | Abcam
Brand: Abcam Limited
SKU: AB181758

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(2 Publications)

Rabbit Polyclonal ARRDC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Arrestin domain-containing protein 1.

View Alternative Names

Arrestin domain-containing protein 1, Alpha-arrestin 1, ARRDC1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Arrestin domain-containing protein 1. The exact immunogen used to generate this antibody is proprietary information.

Q8N5I2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARRDC1 also known as Arrestin Domain Containing 1 functions as an adaptor protein in endocytosis and intracellular signaling processes. It weighs approximately 47 kDa. This protein is expressed in several tissue types including lungs heart and skeletal muscle. It facilitates cellular processes by interacting with members of the NEDD4 family of E3 ubiquitin ligases and plays a role in modulating protein trafficking to lysosomes.

Biological function summary

ARRDC1 acts in positioning ubiquitinated membrane proteins for internalization and subsequent lysosomal degradation. It does not function as part of a large protein complex but interacts with membrane and cytosolic proteins to regulate their locations and fates within the cell. Consequently this protein is essential for maintaining cellular protein homeostasis which can impact fitness of the cell.

Pathways

ARRDC1 engages in the endocytic pathway cooperating with other proteins like TSG101 and CHMP4B of the ESCRT machinery to mediate cargo sorting in the multivesicular bodies. Additionally it influences the ubiquitination pathway by recruiting ubiquitin ligases linking it to pathways responsible for cellular protein turnover and receptor downregulation. These pathways control processes like receptor recycling or degradation stabilizing cellular responses to environmental cues.

ARRDC1 draws associations with conditions such as cancer and Alzheimer's disease. In cancer altered levels of ARRDC1 might affect the turnover of cell surface receptors impacting signaling pathways related to cell proliferation. Moreover disruptions in associated proteins like E3 ubiquitin ligases can play roles in neurodegenerative diseases though the complete connection to Alzheimer's requires more study to fully understand its involvement in protein aggregation or clearance deficits.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (PubMed : 23886940, PubMed : 27462458). Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles (PubMed : 27462458). More generally, plays a role in the extracellular transport of proteins between cells through the release in the extracellular space of microvesicles (PubMed : 22315426). By participating in the ITCH-mediated ubiquitination and subsequent degradation of NOTCH1, negatively regulates the NOTCH signaling pathway (PubMed : 23886940).

See full target information Arrestin domain-containing protein 1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed38785998

2024

Fusion with ARRDC1 or CD63: A Strategy to Enhance p53 Loading into Extracellular Vesicles for Tumor Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Min Liu,Yu Zhang,Jianfeng He,Wanxi Liu,Zhexuan Li,Yiti Zhang,Ao Gu,Mingri Zhao,Mujun Liu,Xionghao Liu

Journal of proteome research 18:947-959 PubMed30608700

2019

Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs.

Applications

Unspecified application

Species

Unspecified reactive species

Lizandra Jimenez,Hui Yu,Andrew J McKenzie,Jeffrey L Franklin,James G Patton,Qi Liu,Alissa M Weaver

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARRDC1 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise