AB64817

Anti-ARRDC3 antibody

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(13 Publications)

Rabbit Polyclonal ARRDC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human ARRDC3.

View Alternative Names

KIAA1376, ARRDC3, Arrestin domain-containing protein 3, TBP-2-like inducible membrane protein, TLIMP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARRDC3 antibody (AB64817)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARRDC3 antibody (AB64817)

IHC image of ab64817 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64817, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-ARRDC3 antibody (AB64817)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ARRDC3 antibody (AB64817)

ICC/IF image of ab64817 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64817, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-ARRDC3 antibody (AB64817)

All lanes:

Western blot - Anti-ARRDC3 antibody (ab64817) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from COLO205 cells at 5 µg

Lane 3:

Extracts from COLO205 cells at 5 µg with immunising peptide

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Western blot - Anti-ARRDC3 antibody (AB64817)

All lanes:

Western blot - Anti-ARRDC3 antibody (ab64817) at 1/500 dilution

Lane 1:

Rat brain lysates

Lane 2:

Mouse brain lysates

Lane 3:

Mouse brain lysates with immunizing peptide

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human ARRDC3. The exact immunogen used to generate this antibody is proprietary information.

Q96B67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab64817 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARRDC3 known as Arrestin Domain Containing 3 is a relatively small protein with a mass of about 47 kDa. It acts by regulating the internalization and degradation of specific seven-transmembrane receptors through endosomal pathways. ARRDC3 expresses widely in various tissues with higher expressions in the brain heart and skeletal muscle. The protein contains an arrestin domain and initiates interactions with other proteins in cellular signaling.

Biological function summary

ARRDC3 influences cellular communication and adapts cellular responses. As part of a complex that includes members of the NEDD4 family E3 ligases it influences ubiquitination processes. ARRDC3 modulates the degradation and downregulation of G-protein-coupled receptors (GPCRs) which plays an important role in maintaining cellular signaling dynamics. This regulation affects multiple cellular functions including receptor recycling and signal transduction efficiency.

Pathways

ARRDC3 prominently integrates into the ubiquitin-proteasome system and GPCR signaling pathways. It functions in pathways that manage receptor trafficking and desensitization. The protein interacts closely with β-arrestins which play significant roles in GPCR internalization. The coordination with these proteins ensures the proper signaling cascade and termination impacting overall pathway efficiency.

Alterations in ARRDC3 expression have implications for cancer and obesity. In certain cancers such as breast cancer altered ARRDC3 levels correlate with tumor progression and metastasis. The protein also associates with energy balance and body weight regulation linking its function to obesity. The interaction with β-arrestins in these disorders suggests a mechanism where disrupted receptor trafficking exacerbates these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed : 20559325, PubMed : 21982743, PubMed : 23208550). Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation (PubMed : 20559325, PubMed : 23208550). May recruit NEDD4 to ADRB2 (PubMed : 20559325). Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes (PubMed : 23208550).

See full target information ARRDC3

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 23:56-69 PubMed38389126

2024

ARRDC3 regulates the targeted therapy sensitivity of clear cell renal cell carcinoma by promoting AXL degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Mulin Chen,Bingde Yin,Yao Liu,Mingzi Li,Suqin Shen,Jiaxue Wu,Weiguo Li,Jie Fan

Head and neck pathology 17:658-672 PubMed37486532

2023

Protease-Activated Receptor 1 (PAR1) Expression Contributes to HPV-Associated Oropharyngeal Cancer Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Fujita,Yujiro Fukuda,Fumiaki Sanuki,Isao Irei,Yasumasa Monobe,Masako Uno,Takeshi Akisada,Koichiro Shimoya,Hirotaka Hara,Takuya Moriya

Molecular reproduction and development 90:406-416 PubMed37436094

2023

Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Long,Shan Wang

Open medicine (Warsaw, Poland) 18:20220546 PubMed37215052

2023

circ_0004904 regulates the trophoblast cell in preeclampsia via miR-19b-3p/ARRDC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyuan Cao,Jie Cui,Guiling Liu

Cancers 10: PubMed30545011

2018

The Role of Arrestin Domain-Containing 3 in Regulating Endocytic Recycling and Extracellular Vesicle Sorting of Integrin β4 in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hwa Soung,Shane Ford,Cecilia Yan,Jun Chung

Oncotarget 8:52935-52947 PubMed28881784

2017

Selective Inhibitors of Nuclear Export (SINE) compounds block proliferation and migration of triple negative breast cancer cells by restoring expression of ARRDC3.

Applications

Unspecified application

Species

Unspecified reactive species

Young Hwa Soung,Trinayan Kashyap,Thalia Nguyen,Garima Yadav,Hua Chang,Yosef Landesman,Jun Chung

The Journal of biological chemistry 291:14510-25 PubMed27226565

2016

The α-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the β2-Adrenergic Receptor.

Applications

Unspecified application

Species

Human

Xufan Tian,Roshanak Irannejad,Shanna L Bowman,Yang Du,Manojkumar A Puthenveedu,Mark von Zastrow,Jeffrey L Benovic

BMC genomics 15:644 PubMed25086961

2014

Prediction of miRNA-mRNA associations in Alzheimer's disease mice using network topology.

Applications

WB, IP

Species

Mouse, Mouse

Haneul Noh,Charny Park,Soojun Park,Young Seek Lee,Soo Young Cho,Hyemyung Seo

Scientific reports 4:3846 PubMed24457910

2014

Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells.

Applications

Species

Human

Young Hwa Soung,Kevin Pruitt,Jun Chung

Journal of cell science 126:4457-68 PubMed23886940

2013

Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals.

Applications

Species

Unspecified reactive species

Loredana Puca,Patricia Chastagner,Vannary Meas-Yedid,Alain Israël,Christel Brou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARRDC3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARRDC3 antibody (AB64817)

Immunocytochemistry/ Immunofluorescence - Anti-ARRDC3 antibody (AB64817)

Western blot - Anti-ARRDC3 antibody (AB64817)

Western blot - Anti-ARRDC3 antibody (AB64817)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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