AB220991

Anti-ARS2 antibody - C-terminal

Name: Anti-ARS2 antibody - C-terminal (ab220991) | Abcam
Brand: Abcam Limited
SKU: AB220991
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal ARS2 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRRT aa 750 to C-terminus.

View Alternative Names

ARS2, ASR2, SRRT, Serrate RNA effector molecule homolog, Arsenite-resistance protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ARS2 antibody - C-terminal (AB220991)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARS2 antibody - C-terminal (AB220991)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling ARS2 with ab220991 at 4 μg/ml (green).

Supplier Data

Western blot - Anti-ARS2 antibody - C-terminal (AB220991)

All lanes:

Western blot - Anti-ARS2 antibody - C-terminal (ab220991) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SRRT aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BXP5

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARS2 also known as SRRT (serrate RNA effector molecule homolog) is a human protein encoded by the SRRT gene. ARS2 is a nuclear protein with an approximate molecular weight of 96 kDa. It is highly expressed in tissues such as the brain lung and liver. Inside the nucleus ARS2 interacts with various RNA-processing complexes binding to nascent RNA transcripts. The primary role of ARS2 centers around RNA metabolism where it manages and organizes the maturation of small RNAs.

Biological function summary

This protein assists in the capping processing and export of several RNA types including pre-miRNAs and other short-length RNAs. ARS2 forms part of a larger protein complex interacting with the Cap Binding Complex (CBC) and Integrator complex which helps regulate RNA stability and processing. Through these interactions ARS2 facilitates the proper maturation and quality control of RNA which is essential for maintaining cellular function and gene expression regulation.

Pathways

The activity of this protein notably aligns with the mRNA processing pathway and RNA surveillance pathway. Within these pathways ARS2 plays a role by participating in the post-transcriptional regulation that is interlinked with proteins like CBC and the nuclear RNA exosome complex. These collaborations enable tight control over RNA homeostasis ensuring that cells can adapt and respond to various cellular signals and stressors by modifying gene expression profiles.

Abnormalities in ARS2 function associate with neural development issues and cancer. In the context of neural development improper ARS2 activity affects RNA processing linking it to neurodevelopmental disorders where the faulty RNA regulation disrupts normal neural gene expression. Its role in cancer relates to unwanted proliferation stemming from misregulated RNA and altered gene expression patterns. Proteins connected to these diseases through ARS2 include DGCR8 (DiGeorge syndrome critical region gene 8) in neurodevelopment and Drosha in various cancers for their shared importance in RNA processing pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity).

See full target information SRRT

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:104289 PubMed35573189

2022

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Iwasaki,Yuka Kawahara,Chikako Okubo,Tatsuya Yamakawa,Michiko Nakamura,Tsuyoshi Tabata,Yohei Nishi,Megumi Narita,Akira Ohta,Hirohide Saito,Takuya Yamamoto,Masato Nakagawa,Shinya Yamanaka,Kazutoshi Takahashi

International journal of molecular medicine 47: PubMed33693954

2021

TGIF1 plays a carcinogenic role in esophageal squamous cell carcinoma through the Wnt/β‑catenin and Akt/mTOR signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Kong,Yang Yu,Hui Guan,Liyang Jiang,Fenghao Sun,Xiaolin Li,Wei Huang,Baosheng Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARS2 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-ARS2 antibody - C-terminal (AB220991)

Western blot - Anti-ARS2 antibody - C-terminal (AB220991)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise