AB192999

Anti-ARS2 antibody - N-terminal

Name: Anti-ARS2 antibody - N-terminal (ab192999) | Abcam
Brand: Abcam Limited
SKU: AB192999

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(4 Publications)

Rabbit Polyclonal ARS2 antibody. N-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SRRT aa 1-50.

View Alternative Names

ARS2, ASR2, SRRT, Serrate RNA effector molecule homolog, Arsenite-resistance protein 2

2 Images

Supplier Data

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (AB192999)

Detection of ARS2 in immunoprecipitates of Jurkat whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192999 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (ab192999)

Predicted band size: 101 kDa

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Supplier Data

Western blot - Anti-ARS2 antibody - N-terminal (AB192999)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ARS2 antibody - N-terminal (ab192999) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 101 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SRRT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BXP5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192999 was affinity purified using an epitope specific to ARS2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARS2 also known as SRRT (serrate RNA effector molecule homolog) is a human protein encoded by the SRRT gene. ARS2 is a nuclear protein with an approximate molecular weight of 96 kDa. It is highly expressed in tissues such as the brain lung and liver. Inside the nucleus ARS2 interacts with various RNA-processing complexes binding to nascent RNA transcripts. The primary role of ARS2 centers around RNA metabolism where it manages and organizes the maturation of small RNAs.

Biological function summary

This protein assists in the capping processing and export of several RNA types including pre-miRNAs and other short-length RNAs. ARS2 forms part of a larger protein complex interacting with the Cap Binding Complex (CBC) and Integrator complex which helps regulate RNA stability and processing. Through these interactions ARS2 facilitates the proper maturation and quality control of RNA which is essential for maintaining cellular function and gene expression regulation.

Pathways

The activity of this protein notably aligns with the mRNA processing pathway and RNA surveillance pathway. Within these pathways ARS2 plays a role by participating in the post-transcriptional regulation that is interlinked with proteins like CBC and the nuclear RNA exosome complex. These collaborations enable tight control over RNA homeostasis ensuring that cells can adapt and respond to various cellular signals and stressors by modifying gene expression profiles.

Abnormalities in ARS2 function associate with neural development issues and cancer. In the context of neural development improper ARS2 activity affects RNA processing linking it to neurodevelopmental disorders where the faulty RNA regulation disrupts normal neural gene expression. Its role in cancer relates to unwanted proliferation stemming from misregulated RNA and altered gene expression patterns. Proteins connected to these diseases through ARS2 include DGCR8 (DiGeorge syndrome critical region gene 8) in neurodevelopment and Drosha in various cancers for their shared importance in RNA processing pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity).

See full target information SRRT

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 94: PubMed32376621

2020

Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Julio C Ruiz,Anne M Devlin,Jiwoong Kim,Nicholas K Conrad

Nature communications 11:2412 PubMed32415063

2020

Conserved protein Pir2 mediates gene repression through cryptic introns in lncRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Gobi Thillainadesan,Hua Xiao,Sahana Holla,Jothy Dhakshnamoorthy,Lisa M Miller Jenkins,David Wheeler,Shiv I S Grewal

Nature communications 11:1063 PubMed32102997

2020

The role of Mediator and Little Elongation Complex in transcription termination.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehisa Takahashi,Amol Ranjan,Shiyuan Chen,Hidefumi Suzuki,Mio Shibata,Tomonori Hirose,Hiroko Hirose,Kazunori Sasaki,Ryota Abe,Kai Chen,Yanfeng He,Ying Zhang,Ichigaku Takigawa,Tadasuke Tsukiyama,Masashi Watanabe,Satoshi Fujii,Midori Iida,Junichi Yamamoto,Yuki Yamaguchi,Yutaka Suzuki,Masaki Matsumoto,Keiichi I Nakayama,Michael P Washburn,Anita Saraf,Laurence Florens,Shigeo Sato,Chieri Tomomori-Sato,Ronald C Conaway,Joan W Conaway,Shigetsugu Hatakeyama

Oncology reports 40:3313-3322 PubMed30542699

2018

Knockdown of arsenic resistance protein 2 inhibits human glioblastoma cell proliferation through the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xue Ke,Yi Pang,Kuijun Chen,Dunke Zhang,Feng Wang,Shunqin Zhu,Jingxin Mao,Xiaosong Hu,Guanghui Zhang,Hongjuan Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ARS2 antibody - N-terminal

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (AB192999)

Western blot - Anti-ARS2 antibody - N-terminal (AB192999)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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