Anti-ARSB antibody [EPR29282-12] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSB antibody [EPR29282-12] - BSA and Azide free (AB326588)

This data was developed using ab323379, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ARSB with ab323379 at 1/100 (5.02 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung carcinoma.
The section was incubated with ab323379 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSB antibody [EPR29282-12] - BSA and Azide free (AB326588)

This data was developed using ab323379, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ARSB with ab323379 at 1/100 (5.02 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon (PMID : 21378286).
The section was incubated with ab323379 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-ARSB antibody [EPR29282-12] - BSA and Azide free (AB326588)

This data was developed using ab323379, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Huh7.

The identity of the bands lower than 60 kDa in lanes 2-4 and lane 7 are unknown.

Lanes 1-5 are incubated with Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/20000 and lanes 6-7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

Lanes 6-7 of this blot were developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 180 seconds; Lanes 4-5 : 37 seconds; Lanes 6-7 : 103 seconds.

All lanes:

Western blot - Anti-ARSB antibody [EPR29282-12] (<a href='/en-us/products/primary-antibodies/arsb-antibody-epr29282-12-ab323379'>ab323379</a>) at 1/1000 dilution

Lane 1:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Human colon tissue lysate at 20 µg

Lane 7:

Human lung cancer tissue lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 6 - 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 70 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-ARSB antibody [EPR29282-12] - BSA and Azide free (AB326588)

This data was developed using ab323379, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the band lower than 60 kDa are unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ARSB antibody [EPR29282-12] (<a href='/en-us/products/primary-antibodies/arsb-antibody-epr29282-12-ab323379'>ab323379</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting ARSB whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-ARSB antibody [EPR29282-12] - BSA and Azide free (AB326588)

This data was developed using ab323379, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody doesn't cross-react with Human ARSI and ARSJ.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (ab244795) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ARSB antibody [EPR29282-12] (<a href='/en-us/products/primary-antibodies/arsb-antibody-epr29282-12-ab323379'>ab323379</a>) at 1/1000 dilution

Lane 1:

His-tagged human ARSB protein fragment at 10 ng

Lane 2:

His-tagged human ARSI recombinant protein at 10 ng

Lane 3:

LIF/His-tagged human ARSJ protein fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,100 kDa

false

Exposure time: 37s