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Rabbit Recombinant Monoclonal ART1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160), expandable thumbnail
  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160), expandable thumbnail
  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160), expandable thumbnail
  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Has ADP-ribosyltransferase activity toward GLP1R.

Alternative names

CD296, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1, ADP-ribosyltransferase C2 and C3 toxin-like 1, Mono(ADP-ribosyl)transferase 1, ARTC1, ART1

Recommended products

Rabbit Recombinant Monoclonal ART1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28754-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316160 is the carrirer-free version of Anti-ART1 antibody [EPR28754-56] ab316159.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ART1 also known as ADP-ribosyltransferase 1 functions mechanically as an enzyme that adds ADP-ribose groups onto arginine residues on target proteins. This post-translational modification plays a role in regulating the function of the substrate proteins. ART1 has a molecular mass approximately around 37 kDa. Expression of ART1 is notable in skeletal muscle and also in various epithelial tissues. The presence of ART1 in these tissues suggests its involvement in several physiological processes.

Biological function summary

ART1 contributes to cellular signaling and substrate protein regulation by altering protein interactions. ART1 does not function as part of a significant large protein complex. However its enzymatic activity impacts the modification and activity of other proteins influencing various cellular responses and processes. These modifications can result in dynamic changes within cellular signaling pathways affecting cellular functions like growth cell cycle and immune response.

Pathways

ART1 participates in pathways related to NAD+ metabolism and ADP-ribosylation. Through these pathways ART1 influences the function of proteins such as integrins and stress response proteins. ART1 by modulating protein activity can affect broader biological processes like cell adhesion and response to cellular stress linked to the pathways it participates in.

Associated diseases and disorders

ART1 relates to conditions such as muscle dystrophy and inflammatory diseases. In muscle dystrophies ART1-mediated ADP-ribosylation affects muscle cell integrity and signaling impacting disease progression. Additionally its interaction with immune proteins like interferon-induced proteins places ART1 as a potential player in inflammatory processes suggesting its involvement in inflammatory diseases. Through these interactions ART1 might contribute to inflammation regulation and tissue integrity in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160), expandable thumbnail

    Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160)

    This data was developed using Anti-ART1 antibody [EPR28754-56] ab316159, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ART1 antibody [EPR28754-56] (Anti-ART1 antibody [EPR28754-56] ab316159) at 1/1000 dilution

    Lane 1: Untreated C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: C2C12 differentiation to muscle for 6 days whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160), expandable thumbnail

    Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160)

    This data was developed using Anti-ART1 antibody [EPR28754-56] ab316159, the same antibody clone in a different buffer formulation.

    Low expression: spleen (PMID: 12070318).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1: 10 seconds, lane 2: 180 seconds

    All lanes: Western blot - Anti-ART1 antibody [EPR28754-56] (Anti-ART1 antibody [EPR28754-56] ab316159) at 1/1000 dilution

    Lane 1: Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 36 kDa

  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160), expandable thumbnail

    Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160)

    This data was developed using Anti-ART1 antibody [EPR28754-56] ab316159, the same antibody clone in a different buffer formulation.

    Low expression: spleen (PMID: 12070318), brain (PMID: 12070318).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ART1 antibody [EPR28754-56] (Anti-ART1 antibody [EPR28754-56] ab316159) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160), expandable thumbnail

    Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (ab316160)

    This data was developed using Anti-ART1 antibody [EPR28754-56] ab316159, the same antibody clone in a different buffer formulation.

    Low expression: spleen (PMID: 12070318).

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ART1 antibody [EPR28754-56] (Anti-ART1 antibody [EPR28754-56] ab316159) at 1/1000 dilution

    Lane 1: Human skeletal muscle tissue lysate at 40 µg with 5% NFDM/TBST

    Lane 2: Human spleen tissue lysate at 40 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 81s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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