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AB316160

Anti-ART1 antibody [EPR28754-56] - BSA and Azide free

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Rabbit Recombinant Monoclonal ART1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

CD296, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1, ADP-ribosyltransferase C2 and C3 toxin-like 1, Mono(ADP-ribosyl)transferase 1, ARTC1, ART1

4 Images
Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)

This data was developed using ab316159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318).

The identity of the higher MW band at approximately 70 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (<a href='/en-us/products/primary-antibodies/art1-antibody-epr28754-56-ab316159'>ab316159</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 40 µg

Lane 2:

Human spleen tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)

This data was developed using ab316159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 10 seconds, lane 2 : 180 seconds

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (<a href='/en-us/products/primary-antibodies/art1-antibody-epr28754-56-ab316159'>ab316159</a>) at 1/1000 dilution

Lane 1:

Rat skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)

This data was developed using ab316159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (<a href='/en-us/products/primary-antibodies/art1-antibody-epr28754-56-ab316159'>ab316159</a>) at 1/1000 dilution

Lane 1:

Untreated C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 2:

C2C12 differentiation to muscle for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] - BSA and Azide free (AB316160)

This data was developed using ab316159, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318), brain (PMID : 12070318).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (<a href='/en-us/products/primary-antibodies/art1-antibody-epr28754-56-ab316159'>ab316159</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28754-56

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316160 is the carrirer-free version of ab316159.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ART1 also known as ADP-ribosyltransferase 1 functions mechanically as an enzyme that adds ADP-ribose groups onto arginine residues on target proteins. This post-translational modification plays a role in regulating the function of the substrate proteins. ART1 has a molecular mass approximately around 37 kDa. Expression of ART1 is notable in skeletal muscle and also in various epithelial tissues. The presence of ART1 in these tissues suggests its involvement in several physiological processes.
Biological function summary

ART1 contributes to cellular signaling and substrate protein regulation by altering protein interactions. ART1 does not function as part of a significant large protein complex. However its enzymatic activity impacts the modification and activity of other proteins influencing various cellular responses and processes. These modifications can result in dynamic changes within cellular signaling pathways affecting cellular functions like growth cell cycle and immune response.

Pathways

ART1 participates in pathways related to NAD+ metabolism and ADP-ribosylation. Through these pathways ART1 influences the function of proteins such as integrins and stress response proteins. ART1 by modulating protein activity can affect broader biological processes like cell adhesion and response to cellular stress linked to the pathways it participates in.

ART1 relates to conditions such as muscle dystrophy and inflammatory diseases. In muscle dystrophies ART1-mediated ADP-ribosylation affects muscle cell integrity and signaling impacting disease progression. Additionally its interaction with immune proteins like interferon-induced proteins places ART1 as a potential player in inflammatory processes suggesting its involvement in inflammatory diseases. Through these interactions ART1 might contribute to inflammation regulation and tissue integrity in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has ADP-ribosyltransferase activity toward GLP1R.
See full target information GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com