AB251155

Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal ART3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TMART, ART3, Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)--arginine ADP-ribosyltransferase 3, ARTC3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

This data was developed using ab192870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain : Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

This data was developed using ab192870, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody

Note : The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).

All lanes:

Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/art3-antibody-epr72202-c-terminal-ab192870'>ab192870</a>)

Predicted band size: 36 kDa,44 kDa

Observed band size: 36 kDa

false

Supplier Data

Western blot - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

This data was developed using ab192870, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ART3 antibody [EPR7220(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/art3-antibody-epr72202-c-terminal-ab192870'>ab192870</a>) at 1/10000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 41 kDa,44 kDa

false

Unconjugated

Anti-ART3 antibody [EPR7220(2)] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7220(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab251155 is the carrier-free version of ab192870.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ART3 also known as ADP-ribosyltransferase 3 is an enzyme with a molecular mass of approximately 41 kDa. It belongs to the family of ARTs (ADP-ribosyltransferases) which facilitate the transfer of ADP-ribose from NAD+ to target proteins modifying their function. ART3 is mainly expressed on the surface of cells with higher expression observed in testicular tissues. Its distribution suggests a specialized role in certain physiological processes.

Biological function summary

ART3 modifies protein functions by adding ADP-ribose groups a process important for cellular signaling and protein interactions. While it is not as deeply investigated as other ART family members ART3 still plays significant roles in certain biological contexts. It operates independently rather than being part of a larger complex. Current research explores its involvement in sperm maturation and other reproductive functions especially due to its elevated expression in testicular tissue.

Pathways

ART3's actions integrate into the wider cellular signaling network influenced by ADP-ribosylation. This modification impacts pathways such as cell communication and possibly apoptosis although detailed mapping in these contexts remains developing. ART3 shares mechanistic similarities with proteins such as ART1 and ART5 which also perform ADP-ribosylation and align with changes in protein activity and signaling dynamics.

ART3 has potential implications in reproductive health and certain cancers. Altered ART3 expression or function may link to testes-related disorders or contribute to cellular oncogenesis. It connects with other proteins like ART1 whose dysregulation has been studied in various cancers. Understanding ART3's contributions could aid in developing targeted treatments or diagnostics related to its roles in these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information ART3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

Western blot - Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free (AB251155)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise