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Rabbit Polyclonal ART5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ART5 aa 1 to C-terminus.

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Images

Western blot - Anti-ART5 antibody (AB151464), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART5 antibody (AB151464), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ART5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96L15

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Alternative names

Recommended products

Rabbit Polyclonal ART5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ART5 aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ART5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96L15
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ART5 also known as ADP-ribosyltransferase 5 is a GPI-anchored cell surface protein. Its approximate mass is around 34 kDa. ART5 expression occurs mainly on the surface of leukocytes and other immune cells indicating its role in immune function. It localizes on the cell membrane which supports its involvement in cell signaling activities.

Biological function summary

ART5 plays a role in the modification of proteins through ADP-ribosylation which is important for signaling and regulatory processes. As part of a larger group of ADP-ribosyltransferases it can modify target proteins by adding ADP-ribose. This modification can affect the activity localization or stability of proteins impacting various cellular processes. Through these actions ART5 contributes to immune responses by influencing cell communication and signal transduction.

Pathways

ART5 influences signaling pathways associated with immune responses. It plays a role in the ADP-ribosylation pathway which involves various immune signaling molecules. Other related proteins involved in this pathway include CD38 and CD157 which also participate in ADP-ribosylation processes. These interactions underline ART5's involvement in regulating immune cell activities and responses.

Associated diseases and disorders

ART5 has been connected to specific immune-related conditions. Its activity or regulation may contribute to autoimmune disorders and inflammatory diseases. ART5 interacts with proteins such as TNF-alpha a cytokine involved in inflammation which hints at its role in modulating inflammatory pathways. Dysregulated ART5 activity could enhance or suppress immune responses affecting how diseases manifest or progress.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-ART5 antibody (ab151464), expandable thumbnail

    Western blot - Anti-ART5 antibody (ab151464)

    10 % SDS PAGE

    All lanes: Western blot - Anti-ART5 antibody (ab151464) at 1/1000 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART5 antibody (ab151464), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART5 antibody (ab151464)

    Immunohistochemical analysis of paraffin-embedded SAS xenograft, labeling ART5 using ab151464 at 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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