JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70800

Anti-ARTD10 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal ARTD10 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PARP10 aa 300-350.

View Alternative Names

Protein mono-ADP-ribosyltransferase PARP10, ADP-ribosyltransferase diphtheria toxin-like 10, Poly [ADP-ribose] polymerase 10, ARTD10, PARP-10, PARP10

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labeling ARTD10 with ab70800 at 1/1000 (1μg/ml). Detection : DAB.

Immunoprecipitation - Anti-ARTD10 antibody (AB70800)
  • IP

Unknown

Immunoprecipitation - Anti-ARTD10 antibody (AB70800)

1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ARTD10 antibody (ab70800)

Predicted band size: 123 kDa

false

Western blot - Anti-ARTD10 antibody (AB70800)
  • WB

Unknown

Western blot - Anti-ARTD10 antibody (AB70800)

All lanes:

Western blot - Anti-ARTD10 antibody (ab70800) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 123 kDa

Observed band size: 123 kDa,60 kDa,70 kDa,90 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human PARP10 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q53GL7

style
left-column

Complementary Products

Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (AB52866)

  • 5
  • 31
Primary Antibodies

AB264221

Anti-USP1 antibody

Immunoprecipitation - Anti-USP1 antibody (AB264221)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ARTD10 also known as PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family. It exhibits a mass of approximately 100 kDa. ARTD10 acts mechanically as mono-ADP-ribosyltransferase which modifies target proteins by adding ADP-ribose units. This specific post-translational modification controls protein function and interactions. ARTD10 expression occurs mostly in the cytoplasm and nucleus across different cell types indicating its versatile functional roles.
Biological function summary

ARTD10 influences several cellular processes including transcriptional regulation and cell cycle progression. It functions independently without being part of a stable protein complex but interacts with other nuclear and cytoplasmic proteins to exert its role. This protein is involved in modulating cellular responses to DNA damage and stress impacting cellular homeostasis and metabolism. Its interaction patterns highlight its significance in cellular regulatory mechanisms.

Pathways

ARTD10 is an active participant in DNA repair and stress response pathways. It is closely linked with the NF-kB signaling pathway where it modulates inflammation and cell survival outcomes. ARTD10 also connects with proteins like PARP1 sharing a broader role in the maintenance of genomic integrity and response to DNA insults. These pathways underpin the critical mechanisms by which cells manage and adapt to damages and maintain cellular viability.

ARTD10's dysregulation associates with certain cancers and inflammatory conditions. Aberrant activity of ARTD10 impacts cellular proliferation and apoptosis linking it with tumorigenesis. Inflammation-related disorders also see a correlation with altered ARTD10 activity. This protein remains related to key proteins like NF-kB in understanding its role in these diseases as they collectively influence inflammatory and tumor progression pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins (PubMed : 18851833, PubMed : 23332125, PubMed : 23474714, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 18851833). Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity (PubMed : 23332125). Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA (PubMed : 24695737).
See full target information PARP10
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and molecular hepatology 31:841-865 PubMed39905842

2025

Glutamate dehydrogenase 1-dependent α-ketoglutarate promotes hepatitis B virus transcription by modulating histone methylations on the covalently closed circular DNA minichromosome.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Tao Cheng,Wei-Xian Chen,Hai-Jun Deng,Xin He,Hui Zhang,Ming Tan,Hai-Bo Yu,Zhen-Zhen Zhang,Ji-Hua Ren,Min-Li Yang,Da-Peng Zhang,Zhi-Hong Li,Juan Chen

Nature communications 15:6197 PubMed39043663

2024

PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jude B Khatib,Ashna Dhoonmoon,George-Lucian Moldovan,Claudia M Nicolae

Frontiers in genetics 13:1035638 PubMed36313419

2022

Depletion of PARP10 inhibits the growth and metastatic potential of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihui Zhou,Bing Wei,Yu Liu,Tian Liu,Sien Zeng,Jinfeng Gan,Guangying Qi
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com