AB178434

Anti-Artemin antibody [EPR9763]

Name: Anti-Artemin antibody [EPR9763] (ab178434) | Abcam
Brand: Abcam Limited
SKU: AB178434
Price: 225 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal Artemin antibody. Suitable for IP, WB and reacts with Rat, Human, Mouse samples. Cited in 8 publications.

View Alternative Names

EVN, ARTN, Artemin, Enovin, Neublastin

2 Images

Lab

Immunoprecipitation - Anti-Artemin antibody [EPR9763] (AB178434)

Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input) : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+) : ab178434 + PC-12 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 23 kDa

All lanes:

Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-Artemin antibody [EPR9763] (AB178434)

All lanes:

Western blot - Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution

Lane 1:

T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 23 kDa

false

Carrier free

Anti-Artemin antibody [EPR9763] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9763

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Artemin also known as ART or ARTN is a neurotrophic factor and part of the glial cell line-derived neurotrophic factor (GDNF) family. It comprises a mature peptide with a molecular mass of approximately 24 kDa. This protein expresses highly in the peripheral and central nervous system particularly in developing and injured tissues. Artemin supports the survival and differentiation of specific neurons contributing to neurite outgrowth and neuronal regeneration.

Biological function summary

Artemin is a significant modulator of neuronal survival and neurite extension. It forms a signaling complex with its receptor components the GDNF family receptor alpha 3 (GFRα3) and the receptor tyrosine kinase (RET). This complex initiates signaling cascades essential for embryonic neuronal development and adult neuroprotection. Artemin selectively activates certain pathways that promote neuronal survival and regenerative responses.

Pathways

Artemin engages with the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/AKT) and the mitogen-activated protein kinase (MAPK/ERK) pathways. These pathways are critical for cell survival proliferation and differentiation. Artemin closely associates with other neurotrophic factors such as GDNF within these pathways suggesting a shared or complementary role in neuroprotection.

Artemin shows potential involvement in neurodegenerative diseases and peripheral neuropathies. It may have therapeutic implications in conditions such as Parkinson's disease where it enhances neuronal survival. Similarly its role in peripheral neuropathy suggests that it modulates pain through interactions with receptors like GFRα3 possibly impacting RET signaling pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Growth factor that supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 10583383, PubMed : 9883723). Acts by binding to its coreceptor, GFRA3, leading to autophosphorylation and activation of the RET receptor (PubMed : 31535977). Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity).

See full target information ARTN

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:1353-1375 PubMed39897524

2025

CLEC11A-Driven Molecular Mechanisms in Intervertebral Disc Degeneration: A Comprehensive Multi-Omics Study.

Applications

Unspecified application

Species

Unspecified reactive species

Nizhou Jiang,Quanxiang Wang,Zhenxin Hu,Xiliang Tian

International journal of molecular sciences 24: PubMed37958856

2023

Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Iwasaki,Koji Akeda,Koki Kawaguchi,Junichi Yamada,Takahiro Hasegawa,Norihiko Takegami,Tatsuhiko Fujiwara,Akihiro Sudo

Frontiers in immunology 14:1202943 PubMed37545522

2023

B lymphocytes transdifferentiate into immunosuppressive erythroblast-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Yang,Zheng Wang,Lei Wu,Ying Wang,Zhihui Xu,Ying Liu,Fangfang Wang,Duonan Yu

Journal of oncology 2022:3332485 PubMed36471885

2022

Artemin Promotes the Migration and Invasion of Cervical Cancer Cells through AKT/mTORC1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjing Zhu,Ling Zhou,Jian Fu,Yijin Wang,Xiaofeng Xu,Jun Wu,Xiangyi Kong,Jian Li,Zhe Zhou,Huaijun Zhou

Science translational medicine 13: PubMed33627484

2021

Radiotherapy and immunotherapy converge on elimination of tumor-promoting erythroid progenitor cells through adaptive immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhu Hou,Hua L Liang,Xinshuang Yu,Zhida Liu,Xuezhi Cao,Enyu Rao,Xiaona Huang,Liangliang Wang,Lei Li,Jason Bugno,Yanbin Fu,Steven J Chmura,Wenjun Wu,Sean Z Luo,Wenxin Zheng,Ainhoa Arina,Jessica Jutzy,Anne R McCall,Everett E Vokes,Sean P Pitroda,Yang-Xin Fu,Ralph R Weichselbaum

International journal of cancer 148:1756-1767 PubMed33236361

2020

Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Jiao Li,Hao Li,Wu-Hu Zhang,Shuai-Shuai Xu,Wang Jiang,Shuo Li,He-Li Gao,Xuan Han,Hua-Xiang Xu,Chun-Tao Wu,Wen-Quan Wang,Xian-Jun Yu,Liang Liu

Thoracic cancer 9:555-562 PubMed29575549

2018

Role of artemin in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zuoqing Song,Fan Yang,Hui Du,Xin Li,Jinghao Liu,Ming Dong,Xiaohong Xu

Experimental cell research 365:12-23 PubMed29453972

2018

Artemin regulates CXCR4 expression to induce migration and invasion in pancreatic cancer cells through activation of NF-κB signaling.

Applications

Unspecified application

Species

Human

Juan Wang,Hui Wang,Jun Cai,Shaoxia Du,Beibei Xin,Wei Wei,Ti Zhang,Xiaohong Shen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Artemin antibody [EPR9763]

Immunoprecipitation - Anti-Artemin antibody [EPR9763] (AB178434)

Western blot - Anti-Artemin antibody [EPR9763] (AB178434)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise