Rabbit Recombinant Monoclonal Artemin antibody. Suitable for IP, WB and reacts with Rat, Human, Mouse samples. Cited in 6 publications.
Images
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] (AB178434), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-ab178434--immunoprecipitation-img4224.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Artemin antibody [EPR9763] (AB178434)")
![Western blot - Anti-Artemin antibody [EPR9763] (AB178434), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-ab178434--western-blot-img5167.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Artemin antibody [EPR9763] (AB178434)")
Publications
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- International journal of molecular sciences 24:2023Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuya Iwasaki,Koji Akeda,Koki Kawaguchi,Junichi Yamada,Takahiro Hasegawa,Norihiko Takegami,Tatsuhiko Fujiwara,Akihiro SudoPubMed 37958856
- Journal of oncology 2022:33324852022Artemin Promotes the Migration and Invasion of Cervical Cancer Cells through AKT/mTORC1 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjing Zhu,Ling Zhou,Jian Fu,Yijin Wang,Xiaofeng Xu,Jun Wu,Xiangyi Kong,Jian Li,Zhe Zhou,Huaijun ZhouPubMed 36471885
- Science translational medicine 13:2021Radiotherapy and immunotherapy converge on elimination of tumor-promoting erythroid progenitor cells through adaptive immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzhu Hou,Hua L Liang,Xinshuang Yu,Zhida Liu,Xuezhi Cao,Enyu Rao,Xiaona Huang,Liangliang Wang,Lei Li,Jason Bugno,Yanbin Fu,Steven J Chmura,Wenjun Wu,Sean Z Luo,Wenxin Zheng,Ainhoa Arina,Jessica Jutzy,Anne R McCall,Everett E Vokes,Sean P Pitroda,Yang-Xin Fu,Ralph R WeichselbaumPubMed 33627484
- International journal of cancer 148:1756-17672020Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian-Jiao Li,Hao Li,Wu-Hu Zhang,Shuai-Shuai Xu,Wang Jiang,Shuo Li,He-Li Gao,Xuan Han,Hua-Xiang Xu,Chun-Tao Wu,Wen-Quan Wang,Xian-Jun Yu,Liang LiuPubMed 33236361
- Thoracic cancer 9:555-5622018Role of artemin in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZuoqing Song et. al.PubMed 29575549
- Experimental cell research 365:12-232018Artemin regulates CXCR4 expression to induce migration and invasion in pancreatic cancer cells through activation of NF-κB signaling.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Growth factor that supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain (PubMed:10583383, PubMed:9883723). Acts by binding to its coreceptor, GFRA3, leading to autophosphorylation and activation of the RET receptor (PubMed:31535977). Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity).
Alternative names
EVN, ARTN, Artemin, Enovin, Neublastin
Recommended products
Rabbit Recombinant Monoclonal Artemin antibody. Suitable for IP, WB and reacts with Rat, Human, Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9763
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Artemin also known as ART or ARTN is a neurotrophic factor and part of the glial cell line-derived neurotrophic factor (GDNF) family. It comprises a mature peptide with a molecular mass of approximately 24 kDa. This protein expresses highly in the peripheral and central nervous system particularly in developing and injured tissues. Artemin supports the survival and differentiation of specific neurons contributing to neurite outgrowth and neuronal regeneration.
- Biological function summary
Artemin is a significant modulator of neuronal survival and neurite extension. It forms a signaling complex with its receptor components the GDNF family receptor alpha 3 (GFRα3) and the receptor tyrosine kinase (RET). This complex initiates signaling cascades essential for embryonic neuronal development and adult neuroprotection. Artemin selectively activates certain pathways that promote neuronal survival and regenerative responses.
- Pathways
Artemin engages with the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/AKT) and the mitogen-activated protein kinase (MAPK/ERK) pathways. These pathways are critical for cell survival proliferation and differentiation. Artemin closely associates with other neurotrophic factors such as GDNF within these pathways suggesting a shared or complementary role in neuroprotection.
- Associated diseases and disorders
Artemin shows potential involvement in neurodegenerative diseases and peripheral neuropathies. It may have therapeutic implications in conditions such as Parkinson's disease where it enhances neuronal survival. Similarly its role in peripheral neuropathy suggests that it modulates pain through interactions with receptors like GFRα3 possibly impacting RET signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-ab178434--immunoprecipitation-img4224.jpg "Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)")
Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)
Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDaAll lanes: Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)
Predicted band size: 23 kDa
![Western blot - Anti-Artemin antibody [EPR9763] (ab178434), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-ab178434--western-blot-img5167.jpg "Western blot - Anti-Artemin antibody [EPR9763] (ab178434)")
Western blot - Anti-Artemin antibody [EPR9763] (ab178434)
All lanes: Western blot - Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution
Lane 1: T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: Rat brain lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
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