Rabbit Recombinant Monoclonal Artemin antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Human, Mouse samples.
Images
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] - BSA and Azide free (AB250054), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-bsa-and-azide-free-ab250054--immunoprecipitation-img4224.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Artemin antibody [EPR9763] - BSA and Azide free (AB250054)")
![Western blot - Anti-Artemin antibody [EPR9763] - BSA and Azide free (AB250054), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-bsa-and-azide-free-ab250054--western-blot-img5167.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Artemin antibody [EPR9763] - BSA and Azide free (AB250054)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Target data
Function
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue.
Alternative names
Artemin, Enovin, Neublastin, EVN, ARTN
Recommended products
Rabbit Recombinant Monoclonal Artemin antibody. Carrier free. Suitable for IP, WB and reacts with Rat, Human, Mouse samples.
Alternative names
Artemin, Enovin, Neublastin, EVN, ARTN
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR9763
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250054 is the carrier-free version of Anti-Artemin antibody [EPR9763] ab178434.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Artemin also known as ART or ARTN is a neurotrophic factor and part of the glial cell line-derived neurotrophic factor (GDNF) family. It comprises a mature peptide with a molecular mass of approximately 24 kDa. This protein expresses highly in the peripheral and central nervous system particularly in developing and injured tissues. Artemin supports the survival and differentiation of specific neurons contributing to neurite outgrowth and neuronal regeneration.
- Biological function summary
Artemin is a significant modulator of neuronal survival and neurite extension. It forms a signaling complex with its receptor components the GDNF family receptor alpha 3 (GFRα3) and the receptor tyrosine kinase (RET). This complex initiates signaling cascades essential for embryonic neuronal development and adult neuroprotection. Artemin selectively activates certain pathways that promote neuronal survival and regenerative responses.
- Pathways
Artemin engages with the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/AKT) and the mitogen-activated protein kinase (MAPK/ERK) pathways. These pathways are critical for cell survival proliferation and differentiation. Artemin closely associates with other neurotrophic factors such as GDNF within these pathways suggesting a shared or complementary role in neuroprotection.
- Associated diseases and disorders
Artemin shows potential involvement in neurodegenerative diseases and peripheral neuropathies. It may have therapeutic implications in conditions such as Parkinson's disease where it enhances neuronal survival. Similarly its role in peripheral neuropathy suggests that it modulates pain through interactions with receptors like GFRα3 possibly impacting RET signaling pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-bsa-and-azide-free-ab250054--immunoprecipitation-img4224.jpg "Immunoprecipitation - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)")
Immunoprecipitation - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)
This data was developed using Anti-Artemin antibody [EPR9763] ab178434, the same antibody clone in a different buffer formulation.
Purified Anti-Artemin antibody [EPR9763] ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): Anti-Artemin antibody [EPR9763] ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Artemin antibody [EPR9763] ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDaAll lanes: Immunoprecipitation - Anti-Artemin antibody [EPR9763] (Anti-Artemin antibody [EPR9763] ab178434)
Predicted band size: 23 kDa
![Western blot - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054), expandable thumbnail](https://content.abcam.com/products/images/artemin-antibody-epr9763-bsa-and-azide-free-ab250054--western-blot-img5167.jpg "Western blot - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)")
Western blot - Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)
This data was developed using Anti-Artemin antibody [EPR9763] ab178434, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Artemin antibody [EPR9763] (Anti-Artemin antibody [EPR9763] ab178434) at 1/1000 dilution
Lane 1: T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: Rat brain lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
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