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AB205564

Anti-Artemin antibody [IF3]

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(2 Publications)

Mouse Monoclonal Artemin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ARTN.

View Alternative Names

EVN, ARTN, Artemin, Enovin, Neublastin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Artemin antibody [IF3] (AB205564)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Artemin antibody [IF3] (AB205564)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue sections from Alzheimer's disease patients labeling Artemin with ab205564 at 5 μg/mL. DAB was used for visualization. Sections were counterstained with toluidine blue.

Immunocytochemistry/ Immunofluorescence - Anti-Artemin antibody [IF3] (AB205564)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Artemin antibody [IF3] (AB205564)

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected with ab205564 at 20 μg/mL. Goat anti-mouse Dylight 488 conjugated antibody (1 μg/mL) was used as secondary antibody. For nuclear staining DAPI was used. Left image; Artemin bound to the GFRa3-Ret51 receptor complex; Right image; Negative control - GFRa3-Ret51 receptor expressing U2OS cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IF3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ARTN.

Q5T4W7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p>Conformational antibody, not suitable for Western Blot application.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.3-20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
IgG fraction purified with Protein G affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Artemin also known as ART or ARTN is a neurotrophic factor and part of the glial cell line-derived neurotrophic factor (GDNF) family. It comprises a mature peptide with a molecular mass of approximately 24 kDa. This protein expresses highly in the peripheral and central nervous system particularly in developing and injured tissues. Artemin supports the survival and differentiation of specific neurons contributing to neurite outgrowth and neuronal regeneration.
Biological function summary

Artemin is a significant modulator of neuronal survival and neurite extension. It forms a signaling complex with its receptor components the GDNF family receptor alpha 3 (GFRα3) and the receptor tyrosine kinase (RET). This complex initiates signaling cascades essential for embryonic neuronal development and adult neuroprotection. Artemin selectively activates certain pathways that promote neuronal survival and regenerative responses.

Pathways

Artemin engages with the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/AKT) and the mitogen-activated protein kinase (MAPK/ERK) pathways. These pathways are critical for cell survival proliferation and differentiation. Artemin closely associates with other neurotrophic factors such as GDNF within these pathways suggesting a shared or complementary role in neuroprotection.

Artemin shows potential involvement in neurodegenerative diseases and peripheral neuropathies. It may have therapeutic implications in conditions such as Parkinson's disease where it enhances neuronal survival. Similarly its role in peripheral neuropathy suggests that it modulates pain through interactions with receptors like GFRα3 possibly impacting RET signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 10583383, PubMed : 9883723). Acts by binding to its coreceptor, GFRA3, leading to autophosphorylation and activation of the RET receptor (PubMed : 31535977). Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (By similarity).
See full target information ARTN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of cancer 148:1756-1767 PubMed33236361

2020

Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Jiao Li,Hao Li,Wu-Hu Zhang,Shuai-Shuai Xu,Wang Jiang,Shuo Li,He-Li Gao,Xuan Han,Hua-Xiang Xu,Chun-Tao Wu,Wen-Quan Wang,Xian-Jun Yu,Liang Liu

Infection and immunity 87: PubMed31182619

2019

Persistent Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Emily G Severance,Raphael P Viscidi,Robert H Yolken,Jianchun Xiao
View all publications
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Product promise

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