AB313837

Anti-Artemis antibody [HL1227] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Artemis antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCLRE1C.

View Alternative Names

ARTEMIS, ASCID, SCIDA, SNM1C, DCLRE1C, Protein artemis, DNA cross-link repair 1C protein, Protein A-SCID, SNM1 homolog C, SNM1-like protein, hSNM1C

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

Immunofluorescent analysis of A431 (Human epidermoid carcinoma cell line) cells labeling Artemis using ab313837 at a 1/500 dilution (green). Blue : Fluoroshield with DAPI. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000. Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

Supplier Data

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

All lanes:

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (ab313837) at 1/1000 dilution

All lanes:

A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 78 kDa

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Lab

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

Western blot : Rabbit Monoclonal[HL1227] to Artemis ab313837 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 80-90 kDa in Wild-type A549 cell lysates with no signal observed at this size in DCLRE1C knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (ab313837) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

DCLRE1C knockout A549 at 20 µg

Lane 3:

Jurkat at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 100 kDa

Observed band size: 80-90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1227

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DCLRE1C.

Q96SD1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Artemis also known as DNA cross-link repair 1C (DCLRE1C) is a protein that plays an important role in DNA repair. It has a molecular mass of approximately 78 kDa. Artemis expresses mainly in cells of the immune system including lymphocytes and has been shown to contribute to maintaining genome stability. The protein acts as a nuclease which means it can cleave phosphodiester bonds within a nucleic acid chain allowing for the resolution of DNA double-strand breaks (DSBs).

Biological function summary

Artemis functions in the resolution of DNA double-strand breaks during processes such as V(D)J recombination and class switch recombination which are essential for the immune system's adaptive response. It forms a complex with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) which is essential for its activation. The complex is necessary for the non-homologous end joining (NHEJ) pathway a major pathway to repair DSBs. The proper functioning of Artemis ensures the integrity and diversity of the antigen receptor repertoire.

Pathways

Artemis plays a critical role in the non-homologous end joining pathway. This pathway is one of the two main mechanisms for repairing double-strand breaks in DNA the other being homologous recombination. Artemis works in conjunction with other proteins such as Ku70/80 and DNA ligase IV in NHEJ. Additionally Artemis's role in V(D)J recombination links it with key immune processes affecting the diversity of antibodies and T-cell receptors important for effective immune responses.

Improper function or mutations in the Artemis protein can lead to severe combined immunodeficiency (SCID) a disorder characterized by a lack of functional immune response. Artemis mutations relate to increased sensitivity to ionizing radiation linking it with radiosensitivity disorders. It also interacts with the protein ATM (ataxia-telangiectasia mutated) which is similarly involved in DNA damage response highlighting a connection between Artemis dysfunction and increased susceptibility to genomic instability-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Nuclease involved in DNA non-homologous end joining (NHEJ); required for double-strand break repair and V(D)J recombination (PubMed : 11336668, PubMed : 11955432, PubMed : 12055248, PubMed : 14744996, PubMed : 15071507, PubMed : 15574326, PubMed : 15936993). Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments (PubMed : 11336668, PubMed : 11955432, PubMed : 14744996). V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs) (PubMed : 11336668, PubMed : 11955432, PubMed : 14744996). These DSBs present two types of DNA end structures : hairpin sealed coding ends and phosphorylated blunt signal ends (PubMed : 11336668, PubMed : 11955432, PubMed : 14744996). These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively (PubMed : 11336668, PubMed : 11955432, PubMed : 14744996). This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC (PubMed : 11955432, PubMed : 15071507, PubMed : 15574326, PubMed : 15936993). The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint (PubMed : 11955432). Also required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ (PubMed : 15456891, PubMed : 15468306, PubMed : 15574327, PubMed : 15811628).

See full target information DCLRE1C

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Artemis antibody [HL1227] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

Western blot - Anti-Artemis antibody [HL1227] - BSA and Azide free (AB313837)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise