Anti-ARTS1/ERAP1 antibody [EPR6069]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1 : 1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

This blot was produced using 4-20% SDS-PAGE containing 15 μg of K-562 whole cell lysate per lane at 150V for 1hr before being transferred onto a 0.45 μm PVDF membrane at 75V for 1hr. The membrane was then blocked for 1hr using 5% NFDM/TBST, then incubated with ab124669 (1/5000) at room temperature for 1hr. After being washed three times in TBST, the membrane was incubated with Peroxidase conjugated goat anti-rabbit IgG (H+L) (ab97051) at 1/20,000 dilution for 1hr at room temperature. The membrane was washed three times again. Then the signal was developed using the ECL technique. ab124669 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in WB. The image shows the band intensity remains relatively constant across all storage temperatures, demonstrating that antibody activity is not affected.

All lanes:

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution

All lanes:

K-562 whole cell lysate at 15 µg with NDFM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 80s

• WB

Lab

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 107 kDa

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• WB

Unknown

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

All lanes:

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP

Predicted band size: 107 kDa

false

• WB

Lab

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution

Lane 1:

Mouse heart lysates at 20 µg

Lane 2:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 107 kDa,122 kDa

Observed band size: 27 kDa

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