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AB232466

Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free

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(4 Publications)

Rabbit Recombinant Monoclonal ARTS1/ERAP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

APPILS, ARTS1, KIAA0525, UNQ584/PRO1154, ERAP1, Endoplasmic reticulum aminopeptidase 1, ARTS-1, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, A-LAP, PILS-AP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (AB232466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (AB232466)

Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (AB232466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (AB232466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1 : 1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6069

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab232466 is the carrier-free version of ab124669.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARTS1 also known as ERAP1 (Endoplasmic Reticulum Aminopeptidase 1) is an aminopeptidase enzyme with a molecular mass of about 110 kDa. This protein is located in the endoplasmic reticulum and plays a significant mechanical role in trimming peptides for presentation by MHC class I molecules. Its expression is highly detectable in cells with robust antigen-presenting capabilities such as dendritic cells and macrophages. Additionally ARTS1/ERAP1 exists in other tissues where immune surveillance is essential.
Biological function summary

The function of ARTS1/ERAP1 involves the processing of proteolytic fragments preparing them for optimal binding to MHC class I molecules. This enzyme participates in a complex alongside ERAP2 another aminopeptidase working cooperatively to adjust peptide lengths to the correct size necessary for MHC class I presentation. Through this role ARTS1/ERAP1 influences the immune system's ability to detect and respond to infected or neoplastic cells by ensuring that antigenic peptides are properly presented.

Pathways

ARTS1/ERAP1 is a critical component of the antigen processing and presentation pathway. This pathway is essential for the presentation of endogenous antigens to cytotoxic T lymphocytes therefore playing a role in adaptive immunity. Related proteins include MHC class I molecules which present the processed peptides and TAP (Transporter associated with Antigen Processing) which transports peptides into the endoplasmic reticulum for further trimming by ARTS1/ERAP1.

Research links ARTS1/ERAP1 to ankylosing spondylitis and other autoimmune diseases. Variations in the ERAP1 gene may affect peptide trimming efficiency altering the peptide repertoire presented by MHC class I molecules and influencing autoimmune activation. The link to another protein HLA-B27 is notable in ankylosing spondylitis as this MHC class I allele frequently associates with the disease highlighting a complex interaction between ARTS1/ERAP1 enzymatic function and autoimmune susceptibility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
See full target information ERAP1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed36009539

2022

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Yadav,Thamil Selvee Ramasamy,Sheng-Che Lin,Szu-Han Chen,Jean Lu,Ya-Hsin Liu,Fu-I Lu,Yuan-Yu Hsueh,Shau-Ping Lin,Chia-Ching Wu

Nutrition, metabolism, and cardiovascular diseases : NMCD 32:2655-2668 PubMed36058761

2022

Quercetin ameliorated insulin resistance via regulating METTL3-mediated N6-methyladenosine modification of PRKD2 mRNA in skeletal muscle and C2C12 myocyte cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiao,Albert Williams,Ning Wei

Journal of neuroinflammation 18:238 PubMed34656124

2021

Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NFκB to promote axonal regeneration and remyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Yadav,Tzu-Chieh Huang,Szu-Han Chen,Thamil Selvee Ramasamy,Yuan-Yu Hsueh,Shau-Ping Lin,Fu-I Lu,Ya-Hsin Liu,Chia-Ching Wu

Frontiers in immunology 11:396 PubMed32210971

2020

The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Seamon,Lesia O Kurlak,Michelle Warthan,Efstratios Stratikos,Jerome F Strauss,Hiten D Mistry,Eun D Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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