Rabbit Recombinant Monoclonal ARVCF antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (AB249723), expandable thumbnail](https://content.abcam.com/products/images/arvcf-antibody-epr100362-bsa-and-azide-free-ab249723--immunocytochemistry-immunofluorescence-img6042.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (AB249723)")
![Western blot - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (AB249723), expandable thumbnail](https://content.abcam.com/products/images/arvcf-antibody-epr100362-bsa-and-azide-free-ab249723--western-blot-img125618.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (AB249723)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Contributes to the regulation of alternative splicing of pre-mRNAs.
Alternative names
Splicing regulator ARVCF, Armadillo repeat protein deleted in velo-cardio-facial syndrome, ARVCF
Recommended products
Rabbit Recombinant Monoclonal ARVCF antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10036(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249723 is the carrier-free version of Anti-ARVCF antibody [EPR10036(2)] ab172633.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARVCF also known as Armadillo Repeat gene deleted in Velo-Cardio-Facial Syndrome encodes a protein involved in cell adhesion and signaling. This protein weighs around 86 kDa and contains armadillo repeat domains facilitating interactions with other membrane-associated proteins. ARVCF is expressed in various tissues including the heart and brain suggesting its versatile role in cellular functioning. Localization in adherens junctions indicates its importance in maintaining structural integrity.
- Biological function summary
The function of ARVCF supports cell adhesion and communication. It participates in complex formation with catenins and cadherins which are critical for adherens junctions. These complexes enable the anchoring of cytoskeletal elements to the cell membrane aiding in stable cell-cell contact. ARVCF modulates the transduction of signals that regulate cellular behaviors such as migration and proliferation through these interactions.
- Pathways
ARVCF plays a role in Wnt signaling and adherens junction pathways. The protein interacts with β-catenin a significant player in Wnt pathway influencing gene expression related to cell growth and differentiation. ARVCF also associates with proteins involved in the assembly and maintenance of adherens junctions further emphasizing its connection to cellular architecture and signaling.
- Associated diseases and disorders
ARVCF has been linked to velo-cardio-facial syndrome and some forms of cancer. These associations suggest a connection between ARVCF dysfunction and disruptions in normal cell signaling and adhesion processes. In cancer altered ARVCF expression can impact the interaction with β-catenin and other junction-related proteins potentially influencing tumor progression and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723), expandable thumbnail](https://content.abcam.com/products/images/arvcf-antibody-epr100362-bsa-and-azide-free-ab249723--immunocytochemistry-immunofluorescence-img6042.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723)")
Immunocytochemistry/ Immunofluorescence - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723)
This data was developed using Anti-ARVCF antibody [EPR10036(2)] ab172633, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARVCF with Anti-ARVCF antibody [EPR10036(2)] ab172633 at a 1/50 dilution. DAPI nuclear staining (blue).
![Western blot - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723), expandable thumbnail](https://content.abcam.com/products/images/arvcf-antibody-epr100362-bsa-and-azide-free-ab249723--western-blot-img125618.jpg "Western blot - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723)")
Western blot - Anti-ARVCF antibody [EPR10036(2)] - BSA and Azide free (ab249723)
This data was developed using Anti-ARVCF antibody [EPR10036(2)] ab172633, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ARVCF antibody [EPR10036(2)] (Anti-ARVCF antibody [EPR10036(2)] ab172633) at 1/1000 dilution
Lane 1: 293T lysate at 10 µg
Lane 2: HeLa lysate at 10 µg
Lane 3: HepG2 lysate at 10 µg
Lane 4: A431 lysate at 10 µg
Predicted band size: 105 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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