Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Immunohistochemical analysis of formalin fixed paraffin embedded mice liver labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Positive staining on mouse liver.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Immunohistochemical analysis of formalin fixed paraffin embedded mice breast carcinoma labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Positive staining on mouse breast carcinoma.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast, Left) / HEPA1-6 (mouse hepatoma epithelial cell, Right) cells labelling Aryl hydrocarbon Receptor with ab309491 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Neuro-2a(PMID : 16956419).

• WB

Lab

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Ahr-KO homozygous mice (Strain ID : T012419).

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (ab309491) at 1/1000 dilution

Lane 1:

Wild-type mouse lung tissue lysate (male) at 20 µg

Lane 2:

Ahr knockout mouse lung tissue lysate (male case1) at 20 µg

Lane 3:

Ahr knockout mouse lung tissue lysate (male case2) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Neuro-2a(PMID : 16956419) Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (ab309491) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 2:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Pancreas In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (ab309491) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse pancreas tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (AB309491)

Immunohistochemical analysis of formalin fixed paraffin embedded mice lung tissue labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Positive staining on (A) Lung tissue from wild-type C57BL/6J mice, no staining on (B) Lung tissue from Ahr knockout mice. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Ahr-KO homozygous mice (Strain ID : T012419).