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Rabbit Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 10 publications.

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Images

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797), expandable thumbnail
  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797), expandable thumbnail
  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Associated Products

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Target data

Function

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed:23275542, PubMed:30373764, PubMed:32818467, PubMed:7961644). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed:23275542, PubMed:30373764, PubMed:7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed:12213388). Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed:7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed:34521881, PubMed:7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed:18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed:32818467, PubMed:32866000). Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed:32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed:28602820). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed:28602820). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).

Alternative names

BHLHE76, AHR, Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76

Recommended products

Rabbit Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7119(N)(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.

Biological function summary

The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.

Pathways

The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.

Associated diseases and disorders

Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797), expandable thumbnail

    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    Lane 1: Wild type HAP1 whole cell lysate (40 μg)
    Lane 2: Empty lane
    Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 μg)
    Lane 4: HeLa whole cell lysate (40 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. ab190797 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/10,000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    Predicted band size: 96 kDa

    Observed band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody at 1/1000 dilution.

    Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

    Counterstained with DAPI (blue). Negative controls used PBS instead of ab190797, then add Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 as the secondary antibody.

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797), expandable thumbnail

    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    All lanes: Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Lane 2: Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa

    Observed band size: 105 kDa

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797), expandable thumbnail

    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    All lanes: Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/1000 dilution

    Lane 1: PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa

    Observed band size: 105 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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