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AB190797

Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

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(17 Publications)

Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 17 publications.

View Alternative Names

BHLHE76, AHR, Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(ab150077) was used as a secondary antibody at 1/1000 dilution.

Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

Counterstained with DAPI (blue). Negative controls used PBS instead of ab190797, then add ab150077 as the secondary antibody.

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)
  • WB

Lab

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : Empty lane
Lane 3 : Aryl hydrocarbon Receptor (KO) whole cell lysate (40 μg)
Lane 4 : HeLa whole cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/10,000 dilution respectively.

Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)
  • WB

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 96 kDa

Observed band size: 105 kDa

false

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)
  • WB

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/1000 dilution

Lane 1:

PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 96 kDa

Observed band size: 105 kDa

false

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • Carrier free

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 660 APC

    APC Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 578 PE

    PE Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • Carrier free

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7119(N)(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB62352

Anti-Nrf2 antibody [EP1808Y]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.
Biological function summary

The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.

Pathways

The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.

Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed : 23275542, PubMed : 30373764, PubMed : 32818467, PubMed : 7961644). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed : 23275542, PubMed : 30373764, PubMed : 7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed : 12213388). Xenobiotics can act as ligands : upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed : 7961644, PubMed : 33193710). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed : 34521881, PubMed : 7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed : 18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed : 32818467, PubMed : 32866000). Acts as a negative regulator of anti-tumor immunity : indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed : 32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed : 28602820). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed : 28602820). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28602820).
See full target information AHR
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:130 PubMed40936027

2025

SP1 and p23 play a crucial role in the circadian target gene induction of activated aryl hydrocarbon receptor in human breast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Melina Mihelakis,Tanina Flore,Gilbert Schönfelder,Michael Oelgeschläger,Norman Ertych

Anais brasileiros de dermatologia 100:501181 PubMed40812224

2025

Expression of the aryl hydrocarbon receptor (AhR) in facial melasma skin compared to healthy perilesional skin.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Nunhez da Silva,Tony Fernando Grassi,Hélio Amante Miot,Ana Cláudia Cavalcante Espósito

iScience 28:112505 PubMed40454092

2025

Polycyclic aromatic hydrocarbons promote tumorigenesis of gallbladder cancer via aryl hydrocarbon Receptor-HEGBC positive feedback axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yuan,Yumeng Zhang,Jianguo Xu,Feng Liu,Yingchao Liang,Beili Wang,Meihong Wu,Zhiyuan Fan,Liang Yang

Toxicological sciences : an official journal of the Society of Toxicology 199:276-288 PubMed38526216

2024

The aryl hydrocarbon receptor differentially modulates the expression profile of antibody isotypes in a human B-cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Mili S Bhakta-Yadav,Kaulini Burra,Nasser Alhamdan,Clayton P Allex-Buckner,Courtney E W Sulentic

Journal for immunotherapy of cancer 11: PubMed37963637

2023

Targeting the aryl hydrocarbon receptor (AhR) with BAY 2416964: a selective small molecule inhibitor for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Kober,Julian Roewe,Norbert Schmees,Lars Roese,Ulrike Roehn,Benjamin Bader,Detlef Stoeckigt,Florian Prinz,Mátyás Gorjánácz,Helge Gottfried Roider,Catherine Olesch,Gabriele Leder,Horst Irlbacher,Ralf Lesche,Julien Lefranc,Mine Oezcan-Wahlbrink,Ankita Sati Batra,Nirmeen Elmadany,Rafael Carretero,Katharina Sahm,Iris Oezen,Frederik Cichon,Daniel Baumann,Ahmed Sadik,Christiane A Opitz,Hilmar Weinmann,Ingo V Hartung,Bertolt Kreft,Rienk Offringa,Michael Platten,Ilona Gutcher

Frontiers in endocrinology 14:1226808 PubMed37664863

2023

AN1284 attenuates steatosis, lipogenesis, and fibrosis in mice with pre-existing non-alcoholic steatohepatitis and directly affects aryl hydrocarbon receptor in a hepatic cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Adi S Yehezkel,Nathalie Abudi,Yuval Nevo,Hadar Benyamini,Sharona Elgavish,Marta Weinstock,Rinat Abramovitch

Cell death discovery 9:269 PubMed37507432

2023

IL4I1-catalyzed tryptophan metabolites mediate the anti-inflammatory function of cytokine-primed human muscle stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muqiu Zuo,Jiankai Fang,Peiqing Huang,Shisong Liu,Pengbo Hou,Shiqing Wang,Zhanhong Liu,Chao Feng,Lijuan Cao,Peishan Li,Yufang Shi,Changshun Shao

Molecular metabolism 66:101617 PubMed36270612

2022

Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyue Li,Yu Yuan,Hongying Chen,Hongwei Dai,Jie Li

Investigational new drugs 40:1216-1230 PubMed36070108

2022

Carbidopa suppresses estrogen receptor-positive breast cancer via AhR-mediated proteasomal degradation of ERα.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Chen,Xing Xia,Heyan Chen,Huirong Huang,Xingsi An,Meng Sun,Qing Yao,Kwonseop Kim,Hailin Zhang,Maoping Chu,Ruijie Chen,Yangzom D Bhutia,Vadivel Ganapathy,Longfa Kou

The Journal of experimental medicine 219: PubMed35977109

2022

A dysregulated sebum-microbial metabolite-IL-33 axis initiates skin inflammation in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoqiong Qiu,Zhenlai Zhu,Xiaochun Liu,Baichao Chen,Huibin Yin,Chaoying Gu,Xiaokai Fang,Ronghui Zhu,Tianze Yu,Wenli Mi,Hong Zhou,Yufeng Zhou,Xu Yao,Wei Li
View all publications
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Product promise

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