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AB232331

Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BHLHE76, AHR, Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(ab150077) was used as a secondary antibody at 1/1000 dilution.

Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

Counterstained with DAPI (blue). Negative controls used PBS instead of ab190797, then add ab150077 as the secondary antibody.

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)
  • WB

Lab

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : Empty lane
Lane 3 : Aryl hydrocarbon Receptor (KO) whole cell lysate (40 μg)
Lane 4 : HeLa whole cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190797).

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (<a href='/en-us/products/primary-antibodies/aryl-hydrocarbon-receptor-antibody-epr7119n2-ab190797'>ab190797</a>)

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

  • Unconjugated

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 578 PE

    PE Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 660 APC

    APC Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

  • Carrier free

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (Detector)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7119(N)(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab232331 is the carrier-free version of ab190797.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.
Biological function summary

The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.

Pathways

The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.

Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed : 23275542, PubMed : 30373764, PubMed : 32818467, PubMed : 7961644). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed : 23275542, PubMed : 30373764, PubMed : 7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed : 12213388). Xenobiotics can act as ligands : upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed : 7961644, PubMed : 33193710). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed : 34521881, PubMed : 7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed : 18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed : 32818467, PubMed : 32866000). Acts as a negative regulator of anti-tumor immunity : indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed : 32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed : 28602820). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed : 28602820). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28602820).
See full target information AHR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:593368 PubMed33362775

2020

Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yu,Dong-Ming Wu,Jing Li,Shi-Hua Deng,Teng Liu,Ting Zhang,Miao He,Yang-Yang Zhao,Ying Xu
View all publications
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Product promise

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