Rabbit Monoclonal Aryl hydrocarbon Receptor antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331), expandable thumbnail](https://content.abcam.com/products/images/aryl-hydrocarbon-receptor-antibody-epr7119n2-bsa-and-azide-free-ab232331--immunocytochemistry-immunofluorescence-img93119.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)")
![Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331), expandable thumbnail](https://content.abcam.com/products/images/aryl-hydrocarbon-receptor-antibody-epr7119n2-bsa-and-azide-free-ab232331--western-blot-img2969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (AB232331)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
7 products for Alternative Version
Target data
Function
Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed:30373764, PubMed:23275542, PubMed:7961644, PubMed:32818467). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed:30373764, PubMed:23275542, PubMed:7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed:12213388). Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed:7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed:7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed:18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed:32866000, PubMed:32818467). Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed:32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed:28602820). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed:28602820). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).
Alternative names
Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, BHLHE76, AHR
Recommended products
Rabbit Monoclonal Aryl hydrocarbon Receptor antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.
Alternative names
Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, BHLHE76, AHR
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR7119(N)(2)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232331 is the carrier-free version of Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.
- Biological function summary
The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.
- Pathways
The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.
- Associated diseases and disorders
Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331), expandable thumbnail](https://content.abcam.com/products/images/aryl-hydrocarbon-receptor-antibody-epr7119n2-bsa-and-azide-free-ab232331--immunocytochemistry-immunofluorescence-img93119.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)")
Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)
Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody at 1/1000 dilution.
Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.
Counterstained with DAPI (blue). Negative controls used PBS instead of Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797, then add Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 as the secondary antibody.
![Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331), expandable thumbnail](https://content.abcam.com/products/images/aryl-hydrocarbon-receptor-antibody-epr7119n2-bsa-and-azide-free-ab232331--western-blot-img2969.jpg "Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)")
Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)
Lane 1: Wild type HAP1 whole cell lysate (40 μg)
Lane 2: Empty lane
Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 μg)
Lane 4: HeLa whole cell lysate (40 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797 observed at 96 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797).
All lanes: Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] ab190797)
Predicted band size: 96 kDa
Observed band size: 96 kDa
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