AB233784

Anti-AS160 antibody [8A11A2]

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(1 Publication)

Mouse Monoclonal AS160 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D4 aa 550-750.

View Alternative Names

AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa

3 Images

Flow Cytometry - Anti-AS160 antibody [8A11A2] (AB233784)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-AS160 antibody [8A11A2] (AB233784)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AS160 with ab233784 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-AS160 antibody [8A11A2] (AB233784)

Expected MW is 41.2 kDa.

All lanes:

Western blot - Anti-AS160 antibody [8A11A2] (ab233784) at 1/500 dilution

All lanes:

Recombinant human AS160 (AA: 574-712) protein

Predicted band size: 146 kDa

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Supplier Data

Western blot - Anti-AS160 antibody [8A11A2] (AB233784)

Lanes 1 - 2:

Western blot - Anti-APC2 antibody [3A2G2] (<a href='/en-us/products/primary-antibodies/apc2-antibody-3a2g2-ab233753'>ab233753</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-AS160 antibody [8A11A2] (ab233784) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

AS160 (AA: 574-712)-hIgGFc transfected HEK-293 whole cell lysate

Predicted band size: 146 kDa,244 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8A11A2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D4 aa 550-750. The exact immunogen used to generate this antibody is proprietary information.

O60343

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.

Biological function summary

AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.

Pathways

AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.

AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

See full target information TBC1D4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:848-856 PubMed38149831

2023

Hypomethylation of the promoter region contributes to the occurrence and development of ovarian endometriosis via the AKT/mTOR/4EBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Qin,Yishuai Li,Yali Hao,Yan Li,Shan Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AS160 antibody [8A11A2]

Flow Cytometry - Anti-AS160 antibody [8A11A2] (AB233784)

Western blot - Anti-AS160 antibody [8A11A2] (AB233784)

Western blot - Anti-AS160 antibody [8A11A2] (AB233784)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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