Rabbit Polyclonal AS160 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TBC1D4 aa 1200 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TBC1D4 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60343
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Alternative names
AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa
Recommended products
Rabbit Polyclonal AS160 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TBC1D4 aa 1200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TBC1D4 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60343
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.
- Biological function summary
AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.
- Pathways
AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.
- Associated diseases and disorders
AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-AS160 antibody (ab134749)
Western Blot Gel 4-8%
All lanes: Western blot - Anti-AS160 antibody (ab134749) at 0.1 µg/mL
Lane 1: Whole cell lysate from HeLa cells at 50 µg
Lane 2: Whole cell lysate from HeLa cells at 15 µg
Lane 3: Whole cell lysate from 293T cells at 50 µg
Lane 4: Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 146 kDa
Exposure time: 10s
Immunoprecipitation - Anti-AS160 antibody (ab134749)
Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 3: Detection of Human AS160 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134749 at 6 μg/mg lysate for IP.
Lane 4: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134749 at 1 μg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.All lanes: Immunoprecipitation - Anti-AS160 antibody (ab134749)
Predicted band size: 146 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com