AB277781

Anti-AS160 (phospho S588) antibody [5HCLC]

Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Multiclonal AS160 phospho S588 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TBC1D4 phospho S588 aa 550-600.

View Alternative Names

AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa

2 Images

Supplier Data

Immunocytochemistry - Anti-AS160 (phospho S588) antibody [5HCLC] (AB277781)

For immunofluorescence analysis HT-29 cells were fixed and permeabilized for detection of endogenous AS160[pS588] using Anti-AS160[pS588] Recombinant Rabbit Multiclonal Antibody (ab277781, 5 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal^™ Secondary Antibody, Alexa Fluor^®® 488 conjugate (1/2000). Nuclei (blue) were stained using SlowFade^® Gold Antifade Mountant with DAPI, and Rhodamine Phalloidin (1/300) was used for cytoskeletal F-actin (red) staining. Detection and localization of AS160[Ps588] (green) in the cytoplasm can be clearly observed in cells treated with IGF (100 ng/mL, 15 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the AS160[Ps588] phospho peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.

Supplier Data

Western blot - Anti-AS160 (phospho S588) antibody [5HCLC] (AB277781)

All lanes:

Western blot - Anti-AS160 (phospho S588) antibody [5HCLC] (ab277781) at 2.5 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with insulin, 30 nmol / L for 15 minutes)

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with IGF1, 100 ng / mL for 15 minutes)

Predicted band size: 146 kDa

true

Exposure time: 15min

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

5HCLC

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human TBC1D4 phospho S588 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

O60343

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.

Biological function summary

AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.

Pathways

AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.

AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

See full target information TBC1D4 phospho S588

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:8099-8115 PubMed39507263

2024

Identifying the ceRNA Regulatory Network in Early-Stage Acute Pancreatitis and Investigating the Therapeutic Potential of NEAT1 in Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Bi Lin,Chaohao Huang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AS160 (phospho S588) antibody [5HCLC]

Immunocytochemistry - Anti-AS160 (phospho S588) antibody [5HCLC] (AB277781)

Western blot - Anti-AS160 (phospho S588) antibody [5HCLC] (AB277781)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise