AB65753

Anti-AS160 (phospho T642) antibody

(1 Review)

(8 Publications)

Sheep Polyclonal AS160 phospho T642 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa

1 Images

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Western blot - Anti-AS160 (phospho T642) antibody (AB65753)

Western blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK-293 cells, basal (-) or with (+) 1 μM insulin treatment for 15 minutes.

All lanes:

Western blot - Anti-AS160 (phospho T642) antibody (ab65753) at 2 µg/mL

Lane 1:

AS160 wild type (WT)

Lane 2:

AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes

Lane 3:

S588A mutant (S588A)

Lane 4:

S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes

Lane 5:

T642A mutant (T642A)

Lane 6:

T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes

Predicted band size: 146 kDa

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Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TBC1D4 aa 600-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60343

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.5 Preservative: 0.05% Sodium azide Constituents: 0.87% Sodium chloride, 0.238% HEPES

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.

Biological function summary

AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.

Pathways

AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.

AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

See full target information TBC1D4 phospho T642

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 152:113270 PubMed35709652

2022

Targeting cholesteryl ester accumulation in the heart improves cardiac insulin response.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Actis Dato,Aleyda Benitez-Amaro,Eduardo Garcia,Lene Claudi,Maria Teresa LaChica Lhoëst,Antoni Iborra,Joan Carles Escola-Gil,Jose Maria Guerra,Valerie Samouillan,Carlos Enrich,Gustavo Chiabrando,Vicenta Llorente-Cortés

The Journal of clinical endocrinology and metabolism 106:e1631-e1646 PubMed33382888

2021

Insulin-Stimulated Muscle Glucose Uptake and Insulin Signaling in Lean and Obese Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Paola A Ramos,Kelli A Lytle,Danae Delivanis,Søren Nielsen,Nathan K LeBrasseur,Michael D Jensen

Phytotherapy research : PTR 34:3236-3248 PubMed32726508

2020

Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruirui Lu,Junqi Chen,Bihao Liu,Hua Lin,Lixia Bai,Peichun Zhang,Dandan Chen,Honglian Li,Jicheng Li,Yu Pang,Yuan Zhou,Jiuyao Zhou,Junbiao Wu

Cells 9: PubMed31936892

2020

LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Actis Dato,Aleyda Benitez-Amaro,David de Gonzalo-Calvo,Maximiliano Vazquez,Gustavo Bonacci,Vicenta Llorente-Cortés,Gustavo Alberto Chiabrando

Molecular medicine reports 17:5821-5829 PubMed29484389

2018

mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Shuai Yang,Xiao-Yan Zhou,Xue-Fang An,Xuan-Jun Liu,Yan-Jun Zhang,Dan Yu

American journal of physiology. Regulatory, integr 311:R307-14 PubMed27280431

2016

Differential glucose metabolism in mice and humans affected by McArdle disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas O Krag,Tomàs Pinós,Tue L Nielsen,Jordi Duran,Mar García-Rocha,Antoni L Andreu,John Vissing

Journal of applied physiology (Bethesda, Md. : 198 113:1852-61 PubMed22936728

2012

Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation.

Applications

Species

Rat

George G Schweitzer,Edward B Arias,Gregory D Cartee

The Journal of biological chemistry 287:16379-89 PubMed22418434

2012

Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high fat diet-induced glucose intolerance.

Applications

Species

Mouse

Fumika Inazuka,Naoyuki Sugiyama,Masaru Tomita,Takaya Abe,Go Shioi,Hiroyasu Esumi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-AS160 (phospho T642) antibody

Western blot - Anti-AS160 (phospho T642) antibody (AB65753)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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