AB131214

Anti-AS160 (phospho T642) antibody [EPR2733(2)]

Name: Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) | Abcam
Brand: Abcam Limited
SKU: AB131214
Price: 395 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal AS160 phospho T642 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 2 publications.

View Alternative Names

AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa

4 Images

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Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

All lanes:

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution

Lane 1:

293T cell lysate - untreated

Lane 2:

293T cell lysate - insulin-treated

Predicted band size: 146 kDa

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Lab

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/mL

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate at 10 µg

Lane 2:

HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (<a href='/en-us/products/biochemicals/calyculin-a-protein-phosphatase-inhibitor-ab141784'>ab141784</a>) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate at 10 µg

Lane 3:

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 146 kDa

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Lab

Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1 : 1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1 : 20000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2733(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.

Biological function summary

AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.

Pathways

AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.

AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

See full target information TBC1D4 phospho T642

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36978970

2023

Inhibiting NLRP3 Inflammasome Activation by CY-09 Helps to Restore Cerebral Glucose Metabolism in 3×Tg-AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangxue Han,Zhijun He,Xia Hu,Xiaoqian Li,Kaixin Zheng,Yingying Huang,Peng Xiao,Qingguo Xie,Jiazuan Ni,Qiong Liu

Journal of applied physiology (Bethesda, Md. : 198 128:350-361 PubMed31895596

2020

Sex and fiber type independently influence AMPK, TBC1D1, and TBC1D4 at rest and during recovery from high-intensity exercise in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Irene S Tobias,Kara K Lazauskas,Jeremy Siu,Pablo B Costa,Jared W Coburn,Andrew J Galpin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AS160 (phospho T642) antibody [EPR2733(2)]

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (AB131214)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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