Skip to main content

Rabbit Recombinant Monoclonal AS160 phospho T642 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (AB218844), expandable thumbnail
  • Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (AB218844), expandable thumbnail
  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (AB218844), expandable thumbnail
  • OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (AB218844), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytDotWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Expected
Tested
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

Alternative names

AS160, KIAA0603, TBC1D4, TBC1 domain family member 4, Akt substrate of 160 kDa

Recommended products

Rabbit Recombinant Monoclonal AS160 phospho T642 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2733(2)
Purification technique
Affinity purification Protein A
Dissociation constant
6.4 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab218844 is the carrier-free version of Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AS160 also known as TBC1D4 is a Rab GTPase-activating protein with a molecular mass of approximately 160 kDa. It is expressed in adipose and muscle tissues where it plays a role in glucose metabolism regulation. AS160 contains vital domains that interact with signaling molecules influencing the membrane trafficking of glucose transporters like GLUT4. These actions facilitate the cellular uptake of glucose which is important for energy production in metabolically active tissues.

Biological function summary

AS160 acts as an important regulator in the insulin signaling pathway. It is associated with a complex network of proteins that modulate GLUT4 translocation to the plasma membrane. The phosphorylation state of AS160 influences its activity thereby impacting the downstream biological processes. Through regulation of intracellular signaling AS160 supports cellular energy homeostasis and its degradation often influenced by ubiquitin-dependent proteolysis can affect its functionality.

Pathways

AS160 acts within the insulin and AMPK signaling pathways. Through these pathways it interacts with proteins such as Akt and AMPK which are important for its activation and subsequent action. Akt phosphorylates AS160 resulting in the release of its inhibitory effects on Rab GTPases. This modification promotes GLUT4 mobilization enhancing glucose uptake during insulin stimulation.

Associated diseases and disorders

AS160 plays a role in conditions like type 2 diabetes and insulin resistance. Dysregulation or mutations within AS160 can disrupt normal glucose metabolism leading to metabolic dysfunctions. In diabetes the interaction between AS160 and Akt becomes impaired hampering the insulin signaling cascade which further exacerbates glucose uptake issues and metabolic diseases. Alterations in AS160 pathway interactions hold potential implications for therapeutic approaches in managing these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844), expandable thumbnail

    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

    This data was developed using Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214) at 1.12 µg/mL

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate at 10 µg

    Lane 2: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (Calyculin A, protein phosphatase inhibitor ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate at 10 µg

    Lane 3: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (Calyculin A, protein phosphatase inhibitor ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the memb at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa

  • Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844), expandable thumbnail

    Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

    This data was developed using Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214, the same antibody clone in a different buffer formulation.Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844), expandable thumbnail

    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

    This data was developed using Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214, the same antibody clone in a different buffer formulation.

    Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

    All lanes: Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (Anti-AS160 (phospho T642) antibody [EPR2733(2)] ab131214) at 1/1000 dilution

    Lane 1: 293T cell lysate - untreated

    Lane 2: 293T cell lysate - insulin-treated

    Predicted band size: 146 kDa

  • OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844), expandable thumbnail

    OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com