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AB113369

Anti-ASAH2 antibody

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(1 Publication)

Rabbit Polyclonal ASAH2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ASAH2.

View Alternative Names

HNAC1, ASAH2, Neutral ceramidase, N-CDase, NCDase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, hCD

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAH2 antibody (AB113369)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAH2 antibody (AB113369)

ab113369 at 5ug/ml staining ASAH2 in formalin-fixed paraffin-embedded Human small intestine by immunohistochemistry.

Western blot - Anti-ASAH2 antibody (AB113369)
  • WB

Unknown

Western blot - Anti-ASAH2 antibody (AB113369)

Lane 1:

Western blot - Anti-ASAH2 antibody (ab113369) at 1 µg/mL

Lane 2:

Western blot - Anti-ASAH2 antibody (ab113369) at 2 µg/mL

All lanes:

293 cell lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ASAH2. The exact immunogen used to generate this antibody is proprietary information.

Q9NR71

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASAH2 also known as N-acylsphingosine amidohydrolase 2 is an important enzyme responsible for the hydrolysis of ceramides into sphingosine and free fatty acids. It has a molecular mass of approximately 85 kDa. ASAH2 is expressed in many tissues with prominent expression observed in the small intestine and brain. Its activity influences cell membrane integrity and intercellular communication which plays an essential role in the normal functioning of cells.
Biological function summary

ASAH2 regulates the levels of ceramide and sphingosine both of which are important lipid signaling molecules. This enzyme exists within cells as part of a complex that maintains lipid homeostasis impacting numerous biological processes through ceramide metabolism. The balance between ceramide and sphingosine signaling is important for cell survival proliferation and apoptosis highlighting the importance of ASAH2 in these cellular dynamics.

Pathways

ASAH2 involves itself in the sphingolipid metabolism pathway which is significant for signal transduction and cellular communication. ASAH2 works closely with proteins like sphingosine kinase which further metabolizes sphingosine into sphingosine-1-phosphate (S1P) a lipid mediator with diverse functions. This pathway plays essential roles in inflammation immune response and cell growth demonstrating the interconnected nature of ASAH2's activity with other critical proteins.

ASAH2 has associations with metabolic disorders and neurodegenerative diseases. Elevated ceramide levels due to ASAH2 dysfunction relate to disorders such as Gaucher disease characterized by abnormal lipid storage and may influence neurodegenerative conditions like Alzheimer's disease. The enzyme interacts with proteins like acid ceramidase where imbalances in these enzymes lead to disruptions in lipid metabolism and contribute to disease pathology making ASAH2 a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed : 10781606, PubMed : 16229686, PubMed : 26190575). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed : 15946935, PubMed : 19345744, PubMed : 24798654). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed : 11278489, PubMed : 17475390). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed : 16061940). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).
See full target information ASAH2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 5:100324 PubMed39803386

2025

Langerhans Cells Directly Interact with Resident T Cells in the Human Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Oka,Tatsuya Hasegawa,Truelian Lee,Valeria S Oliver-Garcia,Mahsa Mortaja,Marjan Azin,Satoshi Horiba,Sabrina S Smith,Sara Khattab,Kathryn E Trerice,Steven T Chen,Yevgeniy R Semenov,Shadmehr Demehri
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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