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AB249617

Anti-ASAH2B antibody [EPR10539] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ASAH2B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ASAH2C, ASAH2L, ASAH2B, Putative inactive neutral ceramidase B, ASAH2-like protein, Putative inactive N-acylsphingosine amidohydrolase 2B, Putative inactive non-lysosomal ceramidase B

3 Images
Flow Cytometry (Intracellular) - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)

This data was developed using ab170949, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells labeling ASAH2C using ab170949 at 1/10 dilution (red) or a rabbit IgG negative (green).

Immunoprecipitation - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)
  • IP

Supplier Data

Immunoprecipitation - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)

This data was developed using ab170949, the same antibody clone in a different buffer formulation.

All lanes:

Immunoprecipitation - Anti-ASAH2B antibody [EPR10539] (<a href='/en-us/products/primary-antibodies/asah2b-antibody-epr10539-ab170949'>ab170949</a>) at 1/10 dilution

All lanes:

HeLa cell lysate at 10 µg

Predicted band size: 19 kDa

false

Western blot - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)
  • WB

Supplier Data

Western blot - Anti-ASAH2B antibody [EPR10539] - BSA and Azide free (AB249617)

This data was developed using ab170949, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ASAH2B antibody [EPR10539] (<a href='/en-us/products/primary-antibodies/asah2b-antibody-epr10539-ab170949'>ab170949</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lystae at 10 µg

Lane 4:

Fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10539

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249617 is the carrier-free version of ab170949.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASAH2B also known as neutral ceramidase B is an enzyme involved in the metabolism of sphingolipids. It has a molecular mass of roughly 100 kDa. The enzyme functions by hydrolyzing ceramides into sphingosine and free fatty acid in a neutral pH environment. ASAH2B is expressed in various tissues including the brain kidney and liver. Its activity contributes significantly to maintaining the balance of sphingolipid-mediated cellular processes.
Biological function summary

ASAH2B impacts cell signaling and microsomal integrity by regulating sphingosine levels. It is not known to be part of a larger complex but it still plays an important role in the ceramide catabolic process. Sphingosine produced by ASAH2B can influence many cellular functions such as apoptosis proliferation and differentiation. It modulates the levels of bioactive lipids impacting cellular homeostasis.

Pathways

The enzyme is integral to the sphingolipid metabolism pathway. By breaking down ceramides ASAH2B facilitates interactions in the sphingolipid signaling pathway which is important for cell fate decisions. ASAH2B works in conjunction with proteins like sphingosine kinase which further converts sphingosine into sphingosine-1-phosphate another signaling molecule essential for cell growth and survival.

ASAH2B dysregulation associates with conditions such as neurodegenerative diseases and cancer. Improper ceramide metabolism influenced by ASAH2B activity may contribute to these pathological states. For instance altered levels of ASAH2B can disrupt normal apoptotic signaling pathways linking it to cancer progression. It also interacts with proteins like ceramide synthase which play roles in these diseases by affecting the balance of pro-apoptotic and pro-growth signals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ASAH2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller
View all publications
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Product promise

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