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AB263968

Anti-ASAP1 / DDEF1 antibody

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(1 Publication)

Rabbit Polyclonal ASAP1 / DDEF1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ASAP1.

View Alternative Names

DDEF1, KIAA1249, PAG2, ASAP1, ADP-ribosylation factor-directed GTPase-activating protein 1, Development and differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP, ARF GTPase-activating protein 1, DEF-1, Differentiation-enhancing factor 1

1 Images
Western blot - Anti-ASAP1 / DDEF1 antibody (AB263968)
  • WB

Supplier Data

Western blot - Anti-ASAP1 / DDEF1 antibody (AB263968)

Primary incubation overnight at 4°C.

Secondary incubation for 45 minutes at RT.

All lanes:

Western blot - Anti-ASAP1 / DDEF1 antibody (ab263968) at 1/1000 dilution

All lanes:

ASAP1 / DDEF1-transfected U118 cell lysate at 35 µg

Secondary

All lanes:

Rabbit secondary HRP at 1/70000 dilution

Predicted band size: 125 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ASAP1. The exact immunogen used to generate this antibody is proprietary information.

Q9ULH1

Specificity

A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity purified antibody is directed against human ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASAP1 also known as DDEF1 stands for ADP-Ribosylation Factor GTPase-Activating Protein 1 and has a molecular weight of approximately 130 kDa. It plays a mechanical role in actin cytoskeleton remodeling by regulating ARF (ADP-ribosylation factor) GTPases. These are involved in intracellular vesicular transport. ASAP1 localizes to the cytoplasm and shows high expression in invasive cancer cell lines with noticeable expression noted in tissues such as the brain liver and lung.
Biological function summary

ASAP1 interacts with components of the focal adhesion complex a group of proteins critical in signaling pathways that connect the extracellular matrix and the actin cytoskeleton. It modulates cell adhesion migration and invasion. ASAP1 binds to proteins like paxillin which further influences the dynamics of actin and membrane trafficking important for cellular rearrangements.

Pathways

ASAP1 plays roles in actin cytoskeleton reorganization pathways and integrin-mediated signaling pathways. These pathways are essential for cell motility and shape. In these contexts ASAP1 interacts with cortactin an actin-binding protein involved in the regulation of cytoskeletal dynamics necessary for the migration and invasion of cancer cells.

ASAP1 has associations with cancer progression particularly in breast and colorectal cancer. Research indicates that elevated ASAP1 expression correlates with aggressive tumor behavior and poor prognosis. Its interaction with paxillin during disease conditions highlights its involvement in tumor invasiveness and metastasis highlighting its potential as a therapeutic target in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation (PubMed : 25673879).
See full target information ASAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human genetics 143:683-694 PubMed38592547

2024

Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kopp,Leonard A Koch,Hristiana Lyubenova,Oliver Küchler,Manuel Holtgrewe,Andranik Ivanov,Christele Dubourg,Erika Launay,Sebastian Brachs,Stefan Mundlos,Nadja Ehmke,Dominik Seelow,Mélanie Fradin,Uwe Kornak,Björn Fischer-Zirnsak
View all publications
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