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AB230841

Anti-ASAP2 antibody

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(1 Publication)

Rabbit Polyclonal ASAP2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ASAP2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DDEF2, KIAA0400, ASAP2, Development and differentiation-enhancing factor 2, Paxillin-associated protein with ARF GAP activity 3, Pyk2 C-terminus-associated protein, PAG3, PAP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP2 antibody (AB230841)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP2 antibody (AB230841)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ASAP2 using ab230841 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP2 antibody (AB230841)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP2 antibody (AB230841)

Formalin-fixed, paraffin-embedded human skeletal muscle (nerve) tissue stained for ASAP2 using ab230841 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ASAP2 antibody (AB230841)
  • WB

Supplier Data

Western blot - Anti-ASAP2 antibody (AB230841)

All lanes:

Western blot - Anti-ASAP2 antibody (ab230841) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

NS-1 whole cell lysate

Lane 3:

H9c2 (rat heart myoblast cell line) whole cell lysate

Predicted band size: 112 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ASAP2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASAP2 also known as PAG3 or DDEF1 acts as an Arf-GAP (GTPase-activating protein) involved in cellular signaling and membrane trafficking. It has a calculated mass of about 115 kDa. Expression of ASAP2 occurs widely across tissues with significant levels in heart brain and skeletal muscle as well as many other tissues to a lesser extent. This protein is an accessory to intracellular processes due to its inherent domain structures allowing interactions with membranes and proteins.
Biological function summary

ASAP2 functions as a regulator of small GTPase signaling which is important for actin cytoskeleton dynamics and vesicle trafficking. It does not function in isolation and forms part of a protein complex with other signaling molecules. This involvement facilitates the coordination of cellular dynamics necessary for processes such as endocytosis and cell migration. The ability of ASAP2 to modulate these pathways highlights its role in orchestrating cellular responses to extracellular stimuli.

Pathways

ASAP2 plays an integral role in the Arf GTPase cycle and cell motility pathways. It interacts with other proteins such as Arf6 influencing cytoskeletal organization and membrane trafficking. The protein's GAP activity ensures that Arf proteins return to their inactive GDP-bound state maintaining cellular homeostasis. This interaction connects ASAP2 to broader regulatory networks that maintain cellular structure and movement.

ASAP2 has associations with cancer particularly influencing metastasis due to its role in cell migration. Aberrant ASAP2 expression may lead to deregulated cell signaling and migration pathways contributing to tumor progression. Additionally this protein has implications in neurological disorders where it interacts with synaptic proteins potentially affecting neuronal connectivity and signaling. These relationships indicate that ASAP2 is not only an important cellular player but also a potential target for therapeutic intervention in relevant diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
See full target information ASAP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 11: PubMed36010498

2022

Si-Wu Water Extracts Protect against Colonic Mucus Barrier Damage by Regulating Muc2 Mucin Expression in Mice Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Ruan,Yujuan Yu,Peiheng Han,Li Zhang,Zhongyi Hu
View all publications
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