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AB187885

Anti-ASAP3 antibody

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(1 Publication)

Rabbit Polyclonal ASAP3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASAP3 aa 650-800.

View Alternative Names

DDEFL1, UPLC1, ASAP3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP3 antibody (AB187885)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP3 antibody (AB187885)

Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue labeling ASPA3 in the cytoplasm of smooth muscle cells with ab187885 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ASAP3 antibody (AB187885)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASAP3 antibody (AB187885)

Immunocytochemical analysis of SiHa (Human cervical carcinoma cell line) whole cells labeling ASAP3 in the nucleoplasm and vesicles with ab187885 at 2 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASAP3 aa 650-800. The exact immunogen used to generate this antibody is proprietary information.

Q8TDY4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ASAP3 also known as AMAP3 is part of the ARF GAP (ADP-ribosylation factor GTPase-activating protein) family. It has a molecular mass of approximately 100 kDa and is known to regulate actin cytoskeleton remodeling. Expression of ASAP3 occurs mostly in epithelial tissues. ASAP3 functions as a GTPase-activating protein for ADP-ribosylation factors and plays a significant role in cellular processes such as migration and adhesion.
Biological function summary

ASAP3 influences cell structure and movement by regulating the dynamics of the actin cytoskeleton. It participates in molecular complexes that manage actin-filaments and microtubule interactions critical for maintaining cell shape and motility. Additionally ASAP3 is essential for directional cell movement especially important during tissue development and repair.

Pathways

ASAP3 impacts cellular processes critical for the regulation of the actin cytoskeleton and cell migration. ASAP3 modulates signaling pathways including the Rho family small GTPases which involve proteins like Rac1 and Cdc42 that coordinate complex cellular movements. These pathways ensure proper cellular responses to external stimuli by orchestrating signaling cascades affecting cell morphology.

ASAP3 is associated with the progression of cancer particularly within the context of increased migration and invasion of tumor cells. Studies highlight its interaction with the protein EGFR (epidermal growth factor receptor) which plays a role in oncogenic pathways. Furthermore alterations in ASAP3 expression have shown links to neurodevelopmental disorders where disruptions in cytoskeleton regulation affect neural cell migration and network formation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes cell proliferation.
See full target information ASAP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 33:1381-1396 PubMed31488579

2019

, the nuclear exosome targeting component, is mutated in familial pulmonary fibrosis and is required for telomerase RNA maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Dustin L Gable,Valeriya Gaysinskaya,Christine C Atik,C Conover Talbot,Byunghak Kang,Susan E Stanley,Elizabeth W Pugh,Nuria Amat-Codina,Kara M Schenk,Murat O Arcasoy,Cory Brayton,Liliana Florea,Mary Armanios
View all publications
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Product promise

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