Rabbit Polyclonal ASAP3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASAP3 aa 650-800.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ASAP3 aa 650-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDY4
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Alternative names
DDEFL1, UPLC1, ASAP3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1
Recommended products
Rabbit Polyclonal ASAP3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASAP3 aa 650-800.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ASAP3 aa 650-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDY4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein ASAP3 also known as AMAP3 is part of the ARF GAP (ADP-ribosylation factor GTPase-activating protein) family. It has a molecular mass of approximately 100 kDa and is known to regulate actin cytoskeleton remodeling. Expression of ASAP3 occurs mostly in epithelial tissues. ASAP3 functions as a GTPase-activating protein for ADP-ribosylation factors and plays a significant role in cellular processes such as migration and adhesion.
- Biological function summary
ASAP3 influences cell structure and movement by regulating the dynamics of the actin cytoskeleton. It participates in molecular complexes that manage actin-filaments and microtubule interactions critical for maintaining cell shape and motility. Additionally ASAP3 is essential for directional cell movement especially important during tissue development and repair.
- Pathways
ASAP3 impacts cellular processes critical for the regulation of the actin cytoskeleton and cell migration. ASAP3 modulates signaling pathways including the Rho family small GTPases which involve proteins like Rac1 and Cdc42 that coordinate complex cellular movements. These pathways ensure proper cellular responses to external stimuli by orchestrating signaling cascades affecting cell morphology.
- Associated diseases and disorders
ASAP3 is associated with the progression of cancer particularly within the context of increased migration and invasion of tumor cells. Studies highlight its interaction with the protein EGFR (epidermal growth factor receptor) which plays a role in oncogenic pathways. Furthermore alterations in ASAP3 expression have shown links to neurodevelopmental disorders where disruptions in cytoskeleton regulation affect neural cell migration and network formation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAP3 antibody (ab187885)
Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue labeling ASPA3 in the cytoplasm of smooth muscle cells with ab187885 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-ASAP3 antibody (ab187885)
Immunocytochemical analysis of SiHa (Human cervical carcinoma cell line) whole cells labeling ASAP3 in the nucleoplasm and vesicles with ab187885 at 2 μg/ml.
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