Rabbit Polyclonal ASB2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASB2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ASB2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q27
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15590664, PubMed:16325183). Mediates Notch-induced ubiquitination and degradation of substrates including TCF3/E2A and JAK2 (PubMed:21119685). Required during embryonic heart development for complete heart looping (By similarity). Required for cardiomyocyte differentiation (PubMed:32179481). Isoform 1. Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA (PubMed:19300455). Also targets DES for proteasomal degradation (By similarity). Acts as a negative regulator of skeletal muscle mass (By similarity). Isoform 2. Targets filamins FLNA and FLNB for proteasomal degradation (PubMed:21737450, PubMed:22916308, PubMed:24044920, PubMed:24052262). This leads to enhanced adhesion of hematopoietic cells to fibronectin (PubMed:21737450). Required for FLNA degradation in immature cardiomyocytes which is necessary for actin cytoskeleton remodeling, leading to proper organization of myofibrils and function of mature cardiomyocytes (By similarity). Required for degradation of FLNA and FLNB in immature dendritic cells (DC) which enhances immature DC migration by promoting DC podosome formation and DC-mediated degradation of the extracellular matrix (By similarity). Does not promote proteasomal degradation of tyrosine-protein kinases JAK1 or JAK2 in hematopoietic cells (PubMed:22916308).
Alternative names
Ankyrin repeat and SOCS box protein 2, ASB-2, ASB2
Recommended products
Rabbit Polyclonal ASB2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASB2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ASB2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q27
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASB2 also known as ankyrin repeat and SOCS box-containing protein 2 plays a role mechanically as part of the E3 ubiquitin ligase complex. It facilitates the tagging of proteins for degradation via the ubiquitin-proteasome system. ASB2 has a molecular mass of approximately 57 kDa and exhibits robust expression in hematopoietic cells particularly within the bone marrow.
- Biological function summary
ASB2 modulates cellular differentiation and it is often associated with lineage determination in hematopoietic cells. As a component of the ubiquitin ligase complex it performs a critical role in regulating protein stability by promoting ubiquitination. ASB2 interacts with several cellular proteins and contributes to the balance of protein degradation ensuring proper cell function and responses to various stimuli.
- Pathways
ASB2 integrates into ubiquitin-mediated proteolysis and hematopoietic cell development pathways. It affects protein turnover and modulates signaling pathways essential for cell cycle regulation. The ASB2 protein interacts with others such as substrates in the ubiquitination process contributing to maintaining cellular homeostasis and promoting normal cellular activities.
- Associated diseases and disorders
ASB2 connections emerge in leukemia and anemia where its dysregulation impacts disease progression. In leukemia altered ASB2 expression disrupts normal cell differentiation suggesting a role in malignant transformation. This protein also associates with components of the proteolysis pathway implicating faulty protein degradation processes in anemia. Understanding ASB2 interactions may provide insights into therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB2 antibody (ab200370)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling ASB2 with ab200370 at a 1/50 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com