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AB200370

Anti-ASB2 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal ASB2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASB2.

View Alternative Names

Ankyrin repeat and SOCS box protein 2, ASB-2, ASB2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB2 antibody (AB200370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB2 antibody (AB200370)

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling ASB2 with ab200370 at a 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ASB2. The exact immunogen used to generate this antibody is proprietary information.

Q96Q27

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASB2 also known as ankyrin repeat and SOCS box-containing protein 2 plays a role mechanically as part of the E3 ubiquitin ligase complex. It facilitates the tagging of proteins for degradation via the ubiquitin-proteasome system. ASB2 has a molecular mass of approximately 57 kDa and exhibits robust expression in hematopoietic cells particularly within the bone marrow.
Biological function summary

ASB2 modulates cellular differentiation and it is often associated with lineage determination in hematopoietic cells. As a component of the ubiquitin ligase complex it performs a critical role in regulating protein stability by promoting ubiquitination. ASB2 interacts with several cellular proteins and contributes to the balance of protein degradation ensuring proper cell function and responses to various stimuli.

Pathways

ASB2 integrates into ubiquitin-mediated proteolysis and hematopoietic cell development pathways. It affects protein turnover and modulates signaling pathways essential for cell cycle regulation. The ASB2 protein interacts with others such as substrates in the ubiquitination process contributing to maintaining cellular homeostasis and promoting normal cellular activities.

ASB2 connections emerge in leukemia and anemia where its dysregulation impacts disease progression. In leukemia altered ASB2 expression disrupts normal cell differentiation suggesting a role in malignant transformation. This protein also associates with components of the proteolysis pathway implicating faulty protein degradation processes in anemia. Understanding ASB2 interactions may provide insights into therapeutic approaches for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 15590664, PubMed : 16325183). Mediates Notch-induced ubiquitination and degradation of substrates including TCF3/E2A and JAK2 (PubMed : 21119685). Required during embryonic heart development for complete heart looping (By similarity). Required for cardiomyocyte differentiation (PubMed : 32179481). Specifically promotes the ubiquitination of SMAD9 and targets it for proteasomal degradation, leading to avoid excessive accumulation of SMAD9 (PubMed : 34845242). Plays a role in the regulation of NK-cell migration by modulating protein levels of filamin A/FLNA via regulation of its ubiquitination and proteasome degradation (By similarity).. Isoform 1. Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA (PubMed : 19300455). Also targets DES for proteasomal degradation (By similarity). Acts as a negative regulator of skeletal muscle mass (By similarity).. Isoform 2. Targets filamins FLNA and FLNB for proteasomal degradation (PubMed : 21737450, PubMed : 22916308, PubMed : 24044920, PubMed : 24052262). This leads to enhanced adhesion of hematopoietic cells to fibronectin (PubMed : 21737450). Required for FLNA degradation in immature cardiomyocytes which is necessary for actin cytoskeleton remodeling, leading to proper organization of myofibrils and function of mature cardiomyocytes (By similarity). Required for degradation of FLNA and FLNB in immature dendritic cells (DC) which enhances immature DC migration by promoting DC podosome formation and DC-mediated degradation of the extracellular matrix (By similarity). Does not promote proteasomal degradation of tyrosine-protein kinases JAK1 or JAK2 in hematopoietic cells (PubMed : 22916308).
See full target information ASB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7324 PubMed39183203

2024

Multi-omics profiling of retinal pigment epithelium reveals enhancer-driven activation of RANK-NFATc1 signaling in traumatic proliferative vitreoretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyu Liao,Xu Zhu,Yumei Lu,Xiaoping Yi,Youhui Hu,Yumeng Zhao,Zhisheng Ye,Xu Guo,Minghui Liang,Xin Jin,Hong Zhang,Xiaohong Wang,Ziming Zhao,Yupeng Chen,Hua Yan
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Product promise

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