AB227475

Anti-ASB8 antibody

Name: Anti-ASB8 antibody (ab227475) | Abcam
Brand: Abcam Limited
SKU: AB227475

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(1 Publication)

Rabbit Polyclonal ASB8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASB8.

View Alternative Names

PP14212, ASB8, Ankyrin repeat and SOCS box protein 8, ASB-8

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ASB8 antibody (AB227475)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASB8 antibody (AB227475)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASB8 (green) using ab227475 at 1/500 dilution in ICC/IF. Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Paraffin-embedded U87 xenograft tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Paraffin-embedded human colon carcinoma tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Paraffin-embedded rat skin tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

Supplier Data

Western blot - Anti-ASB8 antibody (AB227475)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-ASB8 antibody (ab227475) at 1/1000 dilution

All lanes:

IMR32 (human brain neuroblast cell line) whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ASB8. The exact immunogen used to generate this antibody is proprietary information.

Q9H765

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASB8 protein or Ankyrin Repeat and SOCS Box Containing 8 measures approximately 27 kDa in molecular mass. It functions mechanically as part of the SOCS box family which usually interacts with elongin BC complexes. ASB8 contributes to the ubiquitin-proteasome pathway by binding to substrate proteins targeting them for degradation. It is expressed in various human tissues including skeletal muscle and heart indicating its importance in those areas.

Biological function summary

The ASB8 protein modulates different cellular processes by influencing protein turnover. Though ASB8 does not appear as a member of a larger complex it often interacts with elongin B and C to form an E3 ubiquitin ligase complex. This complex facilitates the tagging of proteins with ubiquitin marking them for degradation via the proteasome. By regulating such degradation ASB8 plays roles in maintaining cellular function and homeostasis.

Pathways

ASB8 engages in the ubiquitin-proteasome pathway a significant player in protein quality control and cellular regulation. It also links with signaling pathways like the JAK-STAT pathway impacting cellular responses to external signals. ASB8 is associated with other proteins such as SOCS family members and elongin BC which work together to regulate these pathways. These interactions highlight the regulatory potential of ASB8 in cell signaling and protein turnover.

ASB8 shows connections to muscle-related conditions and cardiac disorders. Mutations or dysregulation of ASB8 could be implicated in muscle atrophy or cardiomyopathies due to its role in muscle and heart tissue. ASB8's involvement in protein degradation and signaling pathways makes it likely to interact with other SOCS family proteins during these disease processes indicating a complex network of interactions influencing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

See full target information ASB8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1633464 PubMed40837015

2025

Chorordin-like 1 inhibits pancreatic cancer cell migration and invasion: involvement of the BMP4/SMAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Yalan Zhong,Yuqiao Song,Hongmei Wang,Zheng Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASB8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ASB8 antibody (AB227475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB8 antibody (AB227475)

Western blot - Anti-ASB8 antibody (AB227475)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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