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AB97918

Anti-ASB9 antibody

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(1 Publication)

Rabbit Polyclonal ASB9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASB9 aa 1 to C-terminus.

View Alternative Names

Ankyrin repeat and SOCS box protein 9, ASB-9, ASB9

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB9 antibody (AB97918)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB9 antibody (AB97918)

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97918 antibody at 1 : 500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ASB9 antibody (AB97918)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASB9 antibody (AB97918)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97918 at 1/200 dilution. Lower image merged with DNA probe.

Western blot - Anti-ASB9 antibody (AB97918)
  • WB

Unknown

Western blot - Anti-ASB9 antibody (AB97918)

12% SDS PAGE

All lanes:

Western blot - Anti-ASB9 antibody (ab97918) at 1/1000 dilution

Lane 1:

HeLaS3 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ASB9 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96DX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASB9 known as Ankyrin Repeat and SOCS Box Protein 9 is a protein that serves significant roles in cellular processes. Its molecular weight is approximately 32 kDa. ASB9 is expressed mainly in kidney and heart tissues. The protein is part of the larger ankyrin repeat and suppressor of cytokine signaling box (SOCS box) protein family which interacts with components of the ubiquitin-proteasome pathway.
Biological function summary

ASB9 regulates protein degradation by modulating the ubiquitination process. It functions as part of an E3 ligase complex facilitating protein tagging for proteasomal degradation. The ubiquitination role of ASB9 affects various substrates within cells modulating processes such as degradation and turnover of proteins therefore controlling cellular homeostasis and responses. This modulatory influence is particularly relevant in cellular stress conditions where precise control of protein levels is necessary.

Pathways

ASB9 plays roles in the ubiquitin-proteasome system and cellular stress response pathways. It interacts within the protein degradation pathways to ensure removal of misfolded or damaged proteins. ASB9 connects with Cullin-RING ubiquitin ligases within this system. Additionally collaborations between ASB9 and heat shock proteins are important for maintaining protein homeostasis contributing to cellular recovery during stress events.

ASB9 has relevance in cardiac and renal dysfunctions. Variations in ASB9 expression or function can perturb proteostasis potentially contributing to heart failure and kidney disease. In the context of heart failure ASB9 may interact with proteins like alpha-actinin influencing cardiac muscle function. For kidney disorders changes in ASB9 levels might alter cellular signaling impacting processes critical for kidney health and filtration function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 25654263, PubMed : 33268465). The ECS(ASB9) complex catalyzes ubiquitination of creatine kinases CKB and CKMT1A (PubMed : 20302626, PubMed : 22418839, PubMed : 25654263, PubMed : 33268465).. Isoform 2. Does not interact with the Elongin BC complex, likely to be a negative regulator of isoform 1.
See full target information ASB9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0212571 PubMed30811458

2019

Gonadotropin regulation of ankyrin-repeat and SOCS-box protein 9 (ASB9) in ovarian follicles and identification of binding partners.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Benoit,Aly Warma,Jacques G Lussier,Kalidou Ndiaye
View all publications
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