AB168811

Anti-ASCC2 antibody

Name: Anti-ASCC2 antibody (ab168811) | Abcam
Brand: Abcam Limited
SKU: AB168811
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ASCC2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ASCC2 aa 700 to C-terminus.

View Alternative Names

ASC1P100, RQT3, ASCC2, Activating signal cointegrator 1 complex subunit 2, ASC-1 complex subunit p100, Trip4 complex subunit p100

2 Images

Unknown

Western blot - Anti-ASCC2 antibody (AB168811)

All lanes:

Western blot - Anti-ASCC2 antibody (ab168811) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

293T whole cell lysate

Lane 3:

Jurkat whole cell lysate

Lane 4:

TCMK-1 whole cell lysate

Lane 5:

NIH3T3 whole cell lysate

Predicted band size: 86 kDa

true

Exposure time: 3min

Unknown

Immunoprecipitation - Anti-ASCC2 antibody (AB168811)

Detection of ASCC2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168811 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ASCC2 antibody (ab168811)

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ASCC2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H1I8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Snake": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as ASCC2 also referred to as Activating Signal Cointegrator 2 Complex Subunit 2 functions mechanically as a regulator in cellular processes. ASCC2 has a molecular mass of approximately 95 kDa. This protein plays a role in transcriptional regulation and is expressed in various tissues including the brain and liver. By interacting with other proteins ASCC2 acts in the modification of gene expression impacting cell behavior and function.

Biological function summary

ASCC2 forms part of the ASCC complex contributing to DNA repair processes and the regulation of transcription. The complex holds an important position in maintaining genomic integrity which is necessary for proper cellular function. Scientists recognize ASCC2 for its role in modulating transcriptional responses to environmental stressors by interacting with specific transcription factors. Its involvement in these processes highlights the importance of ASCC2 in cellular adaptability and resilience.

Pathways

ASCC2 interacts with important biological routes such as the transcription regulation pathway and the DNA damage response pathway. Within these pathways ASCC2 partners with proteins like ASCC1 and ASCC3 working in coordination to regulate cellular stress responses and repair DNA. This interaction highlights ASCC2’s pivotal role in maintaining cellular homeostasis and adaptability to stress conditions.

ASCC2 holds relevance in conditions such as cancer and neurodegenerative disorders. Its interactions can influence pathogenesis and progression in these diseases. The protein links to other figures implicated in these processes such as ASCC1 with ASCC2's expression levels often correlating with disease states. Understanding its mechanisms and interdependencies highlights the potential for ASCC2 as a target in therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes (PubMed : 29144457, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Plays a role in DNA damage repair as component of the ASCC complex (PubMed : 29144457). Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed : 29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed : 12077347). Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes (PubMed : 36302773).

See full target information ASCC2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 81:2808-2822.e10 PubMed34111399

2021

Translation stress and collided ribosomes are co-activators of cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wan,Szymon Juszkiewicz,Daniel Blears,Prashanth Kumar Bajpe,Zhong Han,Peter Faull,Richard Mitter,Aengus Stewart,Ambrosius P Snijders,Ramanujan S Hegde,Jesper Q Svejstrup

Drug design, development and therapy 14:2393-2403 PubMed32606606

2020

Sinomenine Attenuates Acetaminophen-Induced Acute Liver Injury by Decreasing Oxidative Stress and Inflammatory Response via Regulating TGF-β/Smad Pathway in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chen,Yao Wang,Fang-Zhou Jiao,Fan Yang,Xun Li,Lu-Wen Wang

Cell systems 7:627-642.e6 PubMed30471916

2018

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Krystal K Lum,Bokai Song,Joel D Federspiel,Benjamin A Diner,Timothy Howard,Ileana M Cristea

View all publications

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Product promise

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Anti-ASCC2 antibody

Western blot - Anti-ASCC2 antibody (AB168811)

Immunoprecipitation - Anti-ASCC2 antibody (AB168811)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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