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Rabbit Polyclonal ASCC2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASCC2.

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Images

Western blot - Anti-ASCC2 antibody (AB228827), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASCC2 antibody (AB228827), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC2 antibody (AB228827), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ASCC2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1I8

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a role in DNA damage repair as component of the ASCC complex. Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347).

Alternative names

Recommended products

Rabbit Polyclonal ASCC2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASCC2.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ASCC2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1I8
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein known as ASCC2 also referred to as Activating Signal Cointegrator 2 Complex Subunit 2 functions mechanically as a regulator in cellular processes. ASCC2 has a molecular mass of approximately 95 kDa. This protein plays a role in transcriptional regulation and is expressed in various tissues including the brain and liver. By interacting with other proteins ASCC2 acts in the modification of gene expression impacting cell behavior and function.

Biological function summary

ASCC2 forms part of the ASCC complex contributing to DNA repair processes and the regulation of transcription. The complex holds an important position in maintaining genomic integrity which is necessary for proper cellular function. Scientists recognize ASCC2 for its role in modulating transcriptional responses to environmental stressors by interacting with specific transcription factors. Its involvement in these processes highlights the importance of ASCC2 in cellular adaptability and resilience.

Pathways

ASCC2 interacts with important biological routes such as the transcription regulation pathway and the DNA damage response pathway. Within these pathways ASCC2 partners with proteins like ASCC1 and ASCC3 working in coordination to regulate cellular stress responses and repair DNA. This interaction highlights ASCC2’s pivotal role in maintaining cellular homeostasis and adaptability to stress conditions.

Associated diseases and disorders

ASCC2 holds relevance in conditions such as cancer and neurodegenerative disorders. Its interactions can influence pathogenesis and progression in these diseases. The protein links to other figures implicated in these processes such as ASCC1 with ASCC2's expression levels often correlating with disease states. Understanding its mechanisms and interdependencies highlights the potential for ASCC2 as a target in therapeutic strategies for these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-ASCC2 antibody (ab228827), expandable thumbnail

    Western blot - Anti-ASCC2 antibody (ab228827)

    7.5% SDS-PAGE gel.

    All lanes: Western blot - Anti-ASCC2 antibody (ab228827) at 1/5000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 86 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ASCC2 antibody (ab228827), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASCC2 antibody (ab228827)

    Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASCC2 (green) using ab228827 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC2 antibody (ab228827), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC2 antibody (ab228827)

    Paraffin-embedded human breast cancer tissue stained for ASCC2 using ab228827 at 1/500 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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