AB248946

Anti-ASCC2 antibody [EPR9773] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ASCC2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ASC1P100, RQT3, ASCC2, Activating signal cointegrator 1 complex subunit 2, ASC-1 complex subunit p100, Trip4 complex subunit p100

3 Images

Unknown

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

This data was developed using ab150368, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (<a href='/en-us/products/primary-antibodies/ascc2-antibody-epr9773-ab150368'>ab150368</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa

false

Lab

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

This data was developed using ab150368, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (<a href='/en-us/products/primary-antibodies/ascc2-antibody-epr9773-ab150368'>ab150368</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

Lab

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

This data was developed using ab150368, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (<a href='/en-us/products/primary-antibodies/ascc2-antibody-epr9773-ab150368'>ab150368</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Lane 3:

RAW264.7 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

Unconjugated

Anti-ASCC2 antibody [EPR9773]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9773

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248946 is the carrier-free version of ab150368.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as ASCC2 also referred to as Activating Signal Cointegrator 2 Complex Subunit 2 functions mechanically as a regulator in cellular processes. ASCC2 has a molecular mass of approximately 95 kDa. This protein plays a role in transcriptional regulation and is expressed in various tissues including the brain and liver. By interacting with other proteins ASCC2 acts in the modification of gene expression impacting cell behavior and function.

Biological function summary

ASCC2 forms part of the ASCC complex contributing to DNA repair processes and the regulation of transcription. The complex holds an important position in maintaining genomic integrity which is necessary for proper cellular function. Scientists recognize ASCC2 for its role in modulating transcriptional responses to environmental stressors by interacting with specific transcription factors. Its involvement in these processes highlights the importance of ASCC2 in cellular adaptability and resilience.

Pathways

ASCC2 interacts with important biological routes such as the transcription regulation pathway and the DNA damage response pathway. Within these pathways ASCC2 partners with proteins like ASCC1 and ASCC3 working in coordination to regulate cellular stress responses and repair DNA. This interaction highlights ASCC2’s pivotal role in maintaining cellular homeostasis and adaptability to stress conditions.

ASCC2 holds relevance in conditions such as cancer and neurodegenerative disorders. Its interactions can influence pathogenesis and progression in these diseases. The protein links to other figures implicated in these processes such as ASCC1 with ASCC2's expression levels often correlating with disease states. Understanding its mechanisms and interdependencies highlights the potential for ASCC2 as a target in therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes (PubMed : 29144457, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Plays a role in DNA damage repair as component of the ASCC complex (PubMed : 29144457). Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed : 29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed : 12077347). Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes (PubMed : 36302773).

See full target information ASCC2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASCC2 antibody [EPR9773] - BSA and Azide free

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

Western blot - Anti-ASCC2 antibody [EPR9773] - BSA and Azide free (AB248946)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise