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AB122180

Anti-ASCC3 antibody

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(1 Publication)

Rabbit Polyclonal ASCC3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASCC3 aa 150-300.

View Alternative Names

HELIC1, RQT2, ASCC3, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, Trip4 complex subunit p200, ASC1p200

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ASCC3 antibody (AB122180)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASCC3 antibody (AB122180)

Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122180 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC3 antibody (AB122180)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC3 antibody (AB122180)

ab122180 at 1/150 dilution staining ASCC3 in Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ASCC3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8N3C0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASCC3 also known as Activating Signal Cointegrator 1 Complex Subunit 3 is a component of the ASC-1 complex involved in transcription regulation and DNA repair activities. It has a mass of around 145 kDa and is expressed in multiple tissues including the brain and other neuronal tissues. This protein plays an essential mechanical role as a helicase which is involved in the unwinding of DNA and RNA influencing how genetic information is read and processed by the cell.
Biological function summary

ASCC3 contributes to cellular responses to DNA damage. It functions as part of the ASC-1 complex which also includes subunits ASCC1 ASCC2 and TRIP4. This complex participates in recruiting other factors to sites requiring repair thereby maintaining genomic stability. The helicase activity of ASCC3 ensures proper transcript processing affects cellular growth and survival under stress conditions.

Pathways

ASCC3 is involved in DNA damage response and repair pathways. Particularly it engages with the nucleotide excision repair (NER) pathway where it affects how cells respond to UV-induced DNA damage. ASCC3 works in conjunction with proteins like XPA and RPA which share roles in recognizing and repairing damaged DNA. This coordination emphasizes ASCC3's relevance to genomic integrity and response to environmental changes.

ASCC3 has been associated with neurological disorders and cancer. Mutations or dysfunctions in ASCC3 can lead to improper DNA repair which increases susceptibility to cancerous transformations. Additionally ASCC3 shows interaction with other proteins related to neuronal health like ASCC1 and mutations in these proteins have been linked to neurodevelopmental disorders. Accurate study of ASCC3's role could help in understanding disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATPase involved both in DNA repair and rescue of stalled ribosomes (PubMed : 22055184, PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). 3'-5' DNA helicase involved in repair of alkylated DNA : promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions (PubMed : 22055184). Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex (PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed : 12077347).
See full target information ASCC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 64: PubMed38426627

2024

Characterization of KIF20B as a novel prognostic biomarker and therapeutic target for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Regina Wachuka Mbugua,Atsushi Takano,Bayarbat Tsevegjav,Tomoyuki Yokose,Toshinari Yamashita,Yohei Miyagi,Yataro Daigo
View all publications
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