Rabbit Polyclonal ASCC3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASCC3 aa 150-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ASCC3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N3C0
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
ATPase involved both in DNA repair and rescue of stalled ribosomes (PubMed:22055184, PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). 3'-5' DNA helicase involved in repair of alkylated DNA: promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions (PubMed:22055184). Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex (PubMed:28757607, PubMed:32099016, PubMed:32579943, PubMed:36302773). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347).
Alternative names
HELIC1, RQT2, ASCC3, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, Trip4 complex subunit p200, ASC1p200
Recommended products
Rabbit Polyclonal ASCC3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ASCC3 aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ASCC3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N3C0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASCC3 also known as Activating Signal Cointegrator 1 Complex Subunit 3 is a component of the ASC-1 complex involved in transcription regulation and DNA repair activities. It has a mass of around 145 kDa and is expressed in multiple tissues including the brain and other neuronal tissues. This protein plays an essential mechanical role as a helicase which is involved in the unwinding of DNA and RNA influencing how genetic information is read and processed by the cell.
- Biological function summary
ASCC3 contributes to cellular responses to DNA damage. It functions as part of the ASC-1 complex which also includes subunits ASCC1 ASCC2 and TRIP4. This complex participates in recruiting other factors to sites requiring repair thereby maintaining genomic stability. The helicase activity of ASCC3 ensures proper transcript processing affects cellular growth and survival under stress conditions.
- Pathways
ASCC3 is involved in DNA damage response and repair pathways. Particularly it engages with the nucleotide excision repair (NER) pathway where it affects how cells respond to UV-induced DNA damage. ASCC3 works in conjunction with proteins like XPA and RPA which share roles in recognizing and repairing damaged DNA. This coordination emphasizes ASCC3's relevance to genomic integrity and response to environmental changes.
- Associated diseases and disorders
ASCC3 has been associated with neurological disorders and cancer. Mutations or dysfunctions in ASCC3 can lead to improper DNA repair which increases susceptibility to cancerous transformations. Additionally ASCC3 shows interaction with other proteins related to neuronal health like ASCC1 and mutations in these proteins have been linked to neurodevelopmental disorders. Accurate study of ASCC3's role could help in understanding disease mechanisms and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ASCC3 antibody (ab122180)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122180 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCC3 antibody (ab122180)
ab122180 at 1/150 dilution staining ASCC3 in Paraffin-embedded Human Placenta tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com