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AB168810

Anti-ASCC3 antibody

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(2 Publications)

Rabbit Polyclonal ASCC3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ASCC3 aa 2150 to C-terminus.

View Alternative Names

HELIC1, RQT2, ASCC3, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, Trip4 complex subunit p200, ASC1p200

2 Images
Immunoprecipitation - Anti-ASCC3 antibody (AB168810)
  • IP

Unknown

Immunoprecipitation - Anti-ASCC3 antibody (AB168810)

Detection of ASCC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168810 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ASCC3 antibody (ab168810)

Predicted band size: 251 kDa

false

Western blot - Anti-ASCC3 antibody (AB168810)
  • WB

Unknown

Western blot - Anti-ASCC3 antibody (AB168810)

All lanes:

Western blot - Anti-ASCC3 antibody (ab168810) at 1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 251 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ASCC3 aa 2150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N3C0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab168810 was affinity purified using an epitope specific to ASCC3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASCC3 also known as Activating Signal Cointegrator 1 Complex Subunit 3 is a component of the ASC-1 complex involved in transcription regulation and DNA repair activities. It has a mass of around 145 kDa and is expressed in multiple tissues including the brain and other neuronal tissues. This protein plays an essential mechanical role as a helicase which is involved in the unwinding of DNA and RNA influencing how genetic information is read and processed by the cell.
Biological function summary

ASCC3 contributes to cellular responses to DNA damage. It functions as part of the ASC-1 complex which also includes subunits ASCC1 ASCC2 and TRIP4. This complex participates in recruiting other factors to sites requiring repair thereby maintaining genomic stability. The helicase activity of ASCC3 ensures proper transcript processing affects cellular growth and survival under stress conditions.

Pathways

ASCC3 is involved in DNA damage response and repair pathways. Particularly it engages with the nucleotide excision repair (NER) pathway where it affects how cells respond to UV-induced DNA damage. ASCC3 works in conjunction with proteins like XPA and RPA which share roles in recognizing and repairing damaged DNA. This coordination emphasizes ASCC3's relevance to genomic integrity and response to environmental changes.

ASCC3 has been associated with neurological disorders and cancer. Mutations or dysfunctions in ASCC3 can lead to improper DNA repair which increases susceptibility to cancerous transformations. Additionally ASCC3 shows interaction with other proteins related to neuronal health like ASCC1 and mutations in these proteins have been linked to neurodevelopmental disorders. Accurate study of ASCC3's role could help in understanding disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATPase involved both in DNA repair and rescue of stalled ribosomes (PubMed : 22055184, PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). 3'-5' DNA helicase involved in repair of alkylated DNA : promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions (PubMed : 22055184). Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex (PubMed : 28757607, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed : 12077347).
See full target information ASCC3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Functional & integrative genomics 25:103 PubMed40381037

2025

3'UTR RNA editing driven by ADAR1 modulates MDM2 expression in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elanur Almeric,Deniz Karagozoglu,Mustafa Cicek,Didem Naz Dioken,Huseyin Avni Tac,Esra Cicek,Busra Aytul Kirim,Irmak Gurcuoglu,Osman Ugur Sezerman,Nurhan Ozlu,Ayse Elif Erson-Bensan

Cell systems 7:627-642.e6 PubMed30471916

2018

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS.

Applications

Unspecified application

Species

Unspecified reactive species

Krystal K Lum,Bokai Song,Joel D Federspiel,Benjamin A Diner,Timothy Howard,Ileana M Cristea
View all publications
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Product promise

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