AB314100

Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ASCL1 aa 150 to C-terminus.

View Alternative Names

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

7 Images

Supplier Data

Immunocytochemistry - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Detection of human ASCL1/MASH1 in FFPE SHP-77 cells by immunocytochemistry. Antibody : Rabbit antiASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Detection of human ASCL1/MASH1 in FFPE glioblastoma by immunohistochemistry. Antibody : Rabbit antiASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Detection of human ASCL1/MASH1 in FFPE SK-N-Be(2) cells by immunocytochemistry. Antibody : Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SHP-77 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164) used for IP at 6 µl per reaction. ASCL1/MASH1 was also immunoprecipitated by a second antibody against a different epitope of ASCL1/MASH1 (BL-1982A-1E8). For blotting immunoprecipitated ASCL1/MASH1, A700-164 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (ab314100) at 1/1000 dilution

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Detection of mouse ASCL1/MASH1 in FFPE brain by immunohistochemistry. Antibody : Rabbit antiASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Samples : Whole cell lysate (50 µg) from HEK293T, HeLa, SHP-77, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (ab314100) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

SHP-77 Whole cell lysate at 50 µg

Lane 4:

Hep-G2 Whole cell lysate at 50 µg

Lane 5:

MCF-7 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

false

Supplier Data

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, ND7/23, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] (A700-164) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit antiCOPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (ab314100) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Lane 3:

ND7/23 Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

BW5147.3 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR164J

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC, WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ASCL1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P50553

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.

Biological function summary

This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.

Pathways

MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.

MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in neuronal differentiation : acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

See full target information ASCL1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free

Immunocytochemistry - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Immunocytochemistry - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Immunoprecipitation - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Western blot - Anti-ASCL1/MASH1 antibody [BLR164J] - BSA free (AB314100)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise