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AB204348

Anti-ASCT-1 antibody

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(1 Publication)

Rabbit Polyclonal ASCT-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC1A4 aa 450 to C-terminus.

View Alternative Names

ASCT1, SATT, SLC1A4, Neutral amino acid transporter A, Alanine/serine/cysteine/threonine transporter 1, Solute carrier family 1 member 4, ASCT-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ASCT-1 antibody (AB204348)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASCT-1 antibody (AB204348)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling ASCT-1 with ab204348 at 4 μg/ml (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCT-1 antibody (AB204348)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCT-1 antibody (AB204348)

Immunohistochemical analysis of paraffin-embedded Human oral mucosa tissue labeling ASCT-1 with ab204348 at 1/20 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC1A4 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43007

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Alanine Serine Cysteine-preferring Transporter 1 (ASCT-1) also known as SLC1A4 functions as a Na+-dependent transport protein for neutral amino acids including alanine serine and cysteine. It has a molecular mass of approximately 56 kDa. ASCT-1 expresses on the plasma membrane and is found in the brain and the retina. Its role in the transport of certain amino acids is critical for maintaining normal cellular functions and metabolic processes.
Biological function summary

ASCT-1 transports amino acids which contributes to cellular nutrition and neurotransmitter cycling. It does not function as part of a larger protein complex but rather as a standalone transporter. ASCT-1 plays a significant role in neuronal health by regulating amino acids in the synaptic cleft which impacts neurotransmitter synthesis and recycling essential for proper neural communication.

Pathways

ASCT-1 is actively involved in the glutamate cycle which maintains neurotransmitter homeostasis in the brain. Its activity facilitates the uptake of serine used in the synthesis of D-serine an important co-agonist in NMDA receptor pathways. Both the ASCT-2 transporter and SLC1A3 also part of the glutamate transporter family share related functions in these pathways ensuring the synaptic glutamate levels are regulated effectively.

ASCT-1 mutations or dysregulation have links to neurodevelopmental disorders including intellectual disability and epilepsy. Alterations in its function disrupt amino acid transport which can affect neurotransmitter cycling contributing to these conditions. Additionally ASCT-1 shares a connection with SLC1A5 in these disorders where overlapping functions in amino acid transport may exacerbate the symptoms when mutated or dysfunctional.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent neutral amino-acid transporter that mediates transport of alanine, serine, cysteine, proline, hydroxyproline and threonine.
See full target information SLC1A4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36769360

2023

Haploinsufficiency Impairs PI3K/AKT/mTOR/Autophagy Pathway in Human Pluripotent Stem Cells Derived Neural Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junwen Wang,Ziyi Wang,Limeng Dai,Xintong Zhu,Xingying Guan,Junyi Wang,Jia Li,Mao Zhang,Yun Bai,Hong Guo
View all publications
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Product promise

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