Rabbit Polyclonal Asef2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 150-300.
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Publications
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- PLoS genetics 16:e10087212020Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaushin H Waseem et. al.PubMed 32339198
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96N96
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Alternative names
Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2, Asef2, SPATA13
Recommended products
Rabbit Polyclonal Asef2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 150-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96N96
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Asef2 also known as SPATA13 is a Rac-specific guanine nucleotide exchange factor (GEF) that primarily influences actin cytoskeleton dynamics. It has a mass of approximately 91 kDa and contains a DH-PH domain arrangement. Asef2 is expressed in various tissues including the brain kidney and testis. Its role involves modulating the activity of small GTPases particularly Rac1 which are critical for cellular morphological adjustments.
- Biological function summary
In the context of cellular dynamics Asef2 facilitates the reorganization of the cytoskeleton by promoting actin filament assembly and stability. It acts within the context of protein complexes that regulate cell migration and adhesion. Asef2's function influences cellular processes like neurite outgrowth and cell junction assembly thereby supporting cell motility and intercellular communication.
- Pathways
The function of Asef2 integrates into signaling cascades that control cytoskeletal architecture and cell movement. Asef2 prominently interacts with pathways involving the Wnt signaling pathway where it works with proteins such as APC and β-catenin to influence cell division and migration processes. Additionally it plays a role in the integrin signaling pathway coordinating with proteins like paxillin to affect adhesion and motility.
- Associated diseases and disorders
Asef2 displays relevance in cancer metastasis and neurodegenerative disorders. Its aberrant expression associates with the progression of certain cancer types including colorectal cancer where it may enable cancerous cells to invade and metastasize. In neurodegenerative disorders like Alzheimer's disease Asef2's interaction with tau protein suggests its involvement in neuronal dysfunction and degradation. Through these interactions Asef2 may contribute to both the development and progression of these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asef2 antibody (ab122627)
ab122627 at 1/200 dilution staining Asef2 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Asef2 antibody (ab122627)
ab122627 at 4 μg/ml staining Asef2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
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