AB122701

Anti-Asef2 antibody

Name: Anti-Asef2 antibody (ab122701) | Abcam
Brand: Abcam Limited
SKU: AB122701

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(2 Publications)

Rabbit Polyclonal Asef2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 550-650.

View Alternative Names

Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2, Asef2, SPATA13

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Asef2 antibody (AB122701)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Asef2 antibody (AB122701)

Immunofluorescent staining of Human cell line A-431 using ab122701 at 4μg/ml. Antibody staining is shown in green.

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Western blot - Anti-Asef2 antibody (AB122701)

All lanes:

Western blot - Anti-Asef2 antibody (ab122701)

All lanes:

human cell line HDLM-2.

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Spermatogenesis-associated protein 13 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

Q96N96

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asef2 also known as SPATA13 is a Rac-specific guanine nucleotide exchange factor (GEF) that primarily influences actin cytoskeleton dynamics. It has a mass of approximately 91 kDa and contains a DH-PH domain arrangement. Asef2 is expressed in various tissues including the brain kidney and testis. Its role involves modulating the activity of small GTPases particularly Rac1 which are critical for cellular morphological adjustments.

Biological function summary

In the context of cellular dynamics Asef2 facilitates the reorganization of the cytoskeleton by promoting actin filament assembly and stability. It acts within the context of protein complexes that regulate cell migration and adhesion. Asef2's function influences cellular processes like neurite outgrowth and cell junction assembly thereby supporting cell motility and intercellular communication.

Pathways

The function of Asef2 integrates into signaling cascades that control cytoskeletal architecture and cell movement. Asef2 prominently interacts with pathways involving the Wnt signaling pathway where it works with proteins such as APC and β-catenin to influence cell division and migration processes. Additionally it plays a role in the integrin signaling pathway coordinating with proteins like paxillin to affect adhesion and motility.

Asef2 displays relevance in cancer metastasis and neurodegenerative disorders. Its aberrant expression associates with the progression of certain cancer types including colorectal cancer where it may enable cancerous cells to invade and metastasize. In neurodegenerative disorders like Alzheimer's disease Asef2's interaction with tau protein suggests its involvement in neuronal dysfunction and degradation. Through these interactions Asef2 may contribute to both the development and progression of these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.

See full target information Spermatogenesis-associated protein 13

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 147:311-324 PubMed37713627

2023

TRAPPC6B biallelic variants cause a neurodevelopmental disorder with TRAPP II and trafficking disruptions.

Applications

Unspecified application

Species

Unspecified reactive species

Hashem Almousa,Sara A Lewis,Somayeh Bakhtiari,Sandra Hinz Nordlie,Alex Pagnozzi,Helen Magee,Stephanie Efthymiou,Jennifer A Heim,Patricia Cornejo,Maha S Zaki,Najwa Anwar,Shazia Maqbool,Fatima Rahman,Derek E Neilson,Anusha Vemuri,Sheng Chih Jin,Xiao-Ru Yang,Abolfazl Heidari,Koen van Gassen,Aurélien Trimouille,Christel Thauvin-Robinet,James Liu,Ange-Line Bruel,Hoda Tomoum,Mennatallah O Shata,Mais O Hashem,Mehran Beiraghi Toosi,Ehsan Ghayoor Karimiani,Gözde Yeşil,Lokesh Lingappa,Debangana Baruah,Farnoosh Ebrahimzadeh,Julien Van-Gils,Laurence Faivre,Mina Zamani,Hamid Galehdari,Saeid Sadeghian,Gholamreza Shariati,Rahema Mohammad,Jasper van der Smagt,Alya Qari,John B Vincent,A Micheil Innes,Ali Dursun,R Köksal Özgül,Halil Tuna Akar,Kaya Bilguvar,Cyril Mignot,Boris Keren,Claudia Raveli,Lydie Burglen,Alexandra Afenjar,Laura Donker Kaat,Marjon van Slegtenhorst,Fowzan Alkuraya,Henry Houlden,Sergio Padilla-Lopez,Reza Maroofian,Michael Sacher,Michael C Kruer

PLoS genetics 16:e1008721 PubMed32339198

2020

Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Naushin H Waseem,Sancy Low,Amna Z Shah,Deepa Avisetti,Pia Ostergaard,Michael Simpson,Katarzyna A Niemiec,Belen Martin-Martin,Hebah Aldehlawi,Saima Usman,Pak Sang Lee,Anthony P Khawaja,Jonathan B Ruddle,Ameet Shah,Ege Sackey,Alexander Day,Yuzhen Jiang,Geoff Swinfield,Ananth Viswanathan,Giovanna Alfano,Christina Chakarova,Heather J Cordell,David F Garway-Heath,Peng T Khaw,Shomi S Bhattacharya,Ahmad Waseem,Paul J Foster

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Asef2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Asef2 antibody (AB122701)

Western blot - Anti-Asef2 antibody (AB122701)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise