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AB53608

Anti-ASF1 A+B antibody

0

(1 Review)

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(1 Publication)

Rabbit Polyclonal ASF1 antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Drosophila melanogaster asf1.

View Alternative Names

CG9383, asf1, Histone chaperone asf1, Anti-silencing function protein 1, Replication-coupling assembly factor subunit ASF1, dASF1, RCAF subunit ASF1

1 Images
Western blot - Anti-ASF1 A+B antibody (AB53608)
  • WB

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Western blot - Anti-ASF1 A+B antibody (AB53608)

All lanes:

Western blot - Anti-ASF1 A+B antibody (ab53608) at 1/2000 dilution

All lanes:

Drosophila embryo lysate

Predicted band size: 23 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Drosophila melanogaster asf1.

Q9V464

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASF1A and ASF1B also known as Anti-silencing Function 1A and 1B are histone chaperones. They both have a molecular mass around 22 kDa and play an important role in regulating nucleosome assembly by facilitating histone H3-H4 dimer transfer. These proteins are usually expressed in the nucleus of dividing cells and are present in nearly all eukaryotic organisms. They maintain genomic stability and their activity is tightly regulated due to their involvement in the cell cycle.
Biological function summary

ASF1A and ASF1B serve important roles by assisting in DNA replication and repair. They participate in chromatin remodeling and transcriptional regulation through their interaction with other proteins and chromatin complexes. ASF1A and ASF1B function closely with histone H3-H4 tetramers ensuring proper histone storage and supply during S-phase of cell cycle. Their interaction with complexes like the FACT complex helps maintain a dynamic chromatin structure.

Pathways

The histone chaperones ASF1A and ASF1B are integrated into DNA replication and repair pathways. They associate with the DNA damage response pathway as they interact with the ATR/ATM kinase signaling which is important for repairing DNA double-strand breaks. ASF1 proteins also work with chromatin assembly factors to assist the synthesis and stabilization of replication forks. These pathways connect closely with other proteins like CAF-1 which also helps in chromatin assembly during DNA replication.

Disruptions in ASF1A and ASF1B functions have links to cancer and neurodegenerative disorders. Overexpression of ASF1A is observed in certain types of cancer suggesting a role in tumorigenesis through interactions with proteins such as histone H3. Mutations in ASF1B can contribute to neurodegenerative disorders by affecting chromatin structure and function. These disturbances highlight their significant roles and potential as therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 10591219, PubMed : 12381660, PubMed : 14561777, PubMed : 16151251, PubMed : 16396992). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed : 11533245). Plays a role in the formation of silent heterochromatin (PubMed : 12381660, PubMed : 28320872).
See full target information asf1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Plant physiology 162:1164-77 PubMed23596192

2013

ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana D Lario,Elena Ramirez-Parra,Crisanto Gutierrez,Claudia P Spampinato,Paula Casati
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Product promise

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