AB276071

Anti-ASF1b antibody [004]

Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal ASF1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus.

View Alternative Names

Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II, ASF1B

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Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071)

ASF1b was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate using 0.5 μL ab276071 and 60 μg of Immunomagnetic beads Protein G. Western blot was performed from the immunoprecipitate using ab276071 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.

Performed under reducing conditions.

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Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071)

Predicted band size: 22 kDa

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Western blot - Anti-ASF1b antibody [004] (AB276071)

All lanes:

Western blot - Anti-ASF1b antibody [004] (ab276071) at 1/500 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

Secondary

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Rabbit IgG Dylight 800-labeled antibody (H+L) at 1/5000 dilution

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

004

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVP2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.5-2 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASF1b also known as Anti-Silencing Function 1B is a histone chaperone that weighs approximately 22 kDa. This protein is part of the histone fold family facilitating the transfer of histone H3/H4 dimers either into histone octamers or back to the nucleosome. ASF1b is expressed in various human tissues with significant expression in proliferating cells. Its unique ability to influence chromatin assembly positions it as an important player within cellular dynamics.

Biological function summary

The protein assists in the maintenance and regulation of chromatin structure impacting DNA replication and transcription. ASF1b interacts with histone H3/H4 complexes and often works in coordination with CAF-1 forming a multi-protein complex important for nucleosome assembly during DNA replication. Through these interactions ASF1b plays a role in both maintaining genomic stability and facilitating proper cell cycle progression.

Pathways

ASF1b contributes to the DNA replication and repair pathways required for accurate duplication of genetic material. In the DNA damage response ASF1b offers histone supply critical for chromatin structure restoration post-repair. It also interacts with proteins like ATR and CHK1 aiding in the checkpoint response to DNA damage to prevent genomic instability. However its precise regulation within these pathways can have significant cellular consequences related to genome integrity.

The aberrant expression of ASF1b has links to cancer progression most notably in breast and prostate cancers. Overexpression can drive unchecked cell proliferation correlating with aggressive cancer phenotypes. ASF1b's association with oncogenic pathways involves partner proteins such as p53 where ASF1b indirectly influences its tumor suppressor pathway through modulation of chromatin dynamics showcasing its fundamental role in both normal and pathological cell processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524, PubMed : 26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 20953179, PubMed : 21454524, PubMed : 26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Required for gonad development (PubMed : 12842904).

See full target information ASF1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:105 PubMed39844281

2025

Knockout IL4I1 affects macrophages to improve poor efficacy of CD19 CAR-T combined with PD-1 inhibitor in relapsed/refractory diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Yi Zhang,Hairong Xiao,Qingxi Liu,Mingfeng Zhao

World journal of gastrointestinal oncology 14:2353-2366 PubMed36568946

2022

Anti-silencing function 1B knockdown suppresses the malignant phenotype of colorectal cancer by inactivating the phosphatidylinositol 3-kinase/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gen-Hua Yu,Xu-Feng Gong,Ying-Ying Peng,Jun Qian

Neoplasma 69:361-369 PubMed35103478

2022

ASF1B enhances migration and invasion of lung cancers cell via regulating the P53-mediated epithelial-mesenchymal transformation (EMT) signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Lina Xiao,Deng Pan,Lingzhi Hu

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ASF1b antibody [004]

Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071)

Western blot - Anti-ASF1b antibody [004] (AB276071)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise