Rabbit Recombinant Monoclonal ASF1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus.
Images
![Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071), expandable thumbnail](https://content.abcam.com/products/images/asf1b-antibody-004-ab276071--immunoprecipitation-img14656.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071)")
![Western blot - Anti-ASF1b antibody [004] (AB276071), expandable thumbnail](https://content.abcam.com/products/images/asf1b-antibody-004-ab276071--western-blot-img162611.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ASF1b antibody [004] (AB276071)")
Publications
Filter by
- Neoplasma 69:361-3692022ASF1B enhances migration and invasion of lung cancers cell via regulating the P53-mediated epithelial-mesenchymal transformation (EMT) signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Lina Xiao,Deng Pan,Lingzhi HuPubMed 35103478
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NVP2
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 0.5-2 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
Alternative names
Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II, ASF1B
Recommended products
Rabbit Recombinant Monoclonal ASF1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus.
Alternative names
Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II, ASF1B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NVP2
- Clone number
004
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASF1b also known as Anti-Silencing Function 1B is a histone chaperone that weighs approximately 22 kDa. This protein is part of the histone fold family facilitating the transfer of histone H3/H4 dimers either into histone octamers or back to the nucleosome. ASF1b is expressed in various human tissues with significant expression in proliferating cells. Its unique ability to influence chromatin assembly positions it as an important player within cellular dynamics.
- Biological function summary
The protein assists in the maintenance and regulation of chromatin structure impacting DNA replication and transcription. ASF1b interacts with histone H3/H4 complexes and often works in coordination with CAF-1 forming a multi-protein complex important for nucleosome assembly during DNA replication. Through these interactions ASF1b plays a role in both maintaining genomic stability and facilitating proper cell cycle progression.
- Pathways
ASF1b contributes to the DNA replication and repair pathways required for accurate duplication of genetic material. In the DNA damage response ASF1b offers histone supply critical for chromatin structure restoration post-repair. It also interacts with proteins like ATR and CHK1 aiding in the checkpoint response to DNA damage to prevent genomic instability. However its precise regulation within these pathways can have significant cellular consequences related to genome integrity.
- Associated diseases and disorders
The aberrant expression of ASF1b has links to cancer progression most notably in breast and prostate cancers. Overexpression can drive unchecked cell proliferation correlating with aggressive cancer phenotypes. ASF1b's association with oncogenic pathways involves partner proteins such as p53 where ASF1b indirectly influences its tumor suppressor pathway through modulation of chromatin dynamics showcasing its fundamental role in both normal and pathological cell processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071), expandable thumbnail](https://content.abcam.com/products/images/asf1b-antibody-004-ab276071--immunoprecipitation-img14656.jpg "Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071)")
Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071)
ASF1b was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate using 0.5 μL ab276071 and 60 μg of Immunomagnetic beads Protein G. Western blot was performed from the immunoprecipitate using ab276071 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.
All lanes: Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071)
Predicted band size: 22 kDa
![Western blot - Anti-ASF1b antibody [004] (ab276071), expandable thumbnail](https://content.abcam.com/products/images/asf1b-antibody-004-ab276071--western-blot-img162611.jpg "Western blot - Anti-ASF1b antibody [004] (ab276071)")
Western blot - Anti-ASF1b antibody [004] (ab276071)
All lanes: Western blot - Anti-ASF1b antibody [004] (ab276071) at 1/500 dilution
All lanes: K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg
SecondaryAll lanes: Rabbit IgG Dylight 800-labeled antibody (H+L) at 1/5000 dilution
Predicted band size: 22 kDa
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